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首页> 外文期刊>African Journal of Biotechnology >Effect of medium composition and ultrasonication on xylanase production by Trichoderma harzianum MTCC 4358 on novel substrate
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Effect of medium composition and ultrasonication on xylanase production by Trichoderma harzianum MTCC 4358 on novel substrate

机译：培养基组成和超声处理对新型底物哈茨木霉MTCC 4358产生木聚糖酶的影响

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摘要

Sunflower sludge was used as the sole carbon source for xylanase production, an important industrial enzyme used in pulp and paper industry, by the fungi?Trichoderma harzianum?MTCC 4358. Sunflower sludge was subjected to alkaline pretreatment which showed higher enzyme activity than?the?substrate used without pretreatment. The effect and significance of the medium components were studied using the Plackett-Burman design. The experimental xylanase activity was subjected to statistical analysis using MINITAB 15 software. It was found that peptone, urea, CaCl2.2H2O, Tween 80,?(NH4)2.SO4,?MgSO4.7H2O?and?MnSO4?significantly influenced xylanase production (8.63 U/g of dry substrate). The enzyme showed maximum activity at pH 5 (8.75 U/g of dry substrate), 45°C (8.66 U/g of dry substrate) and 80% initial moisture content (8.84 U/g of dry substrate) and a period of 7 days incubation (8.57 U/g of dry substrate) was necessary for maximum enzyme production. Under these optimized physical conditions and in optimized medium?T. harzianum?MTCC 4358 was inoculated and subjected to ultrasonication for every 12 h (for a period of 8 days), which resulted in higher enzymatic activity of 9.27 U/g of dry substrate.
机译：向日葵污泥被木霉哈茨木霉菌MTCC 4358用作木聚糖酶生产的唯一碳源，木聚糖酶是制浆和造纸工业中的重要工业酶。向日葵污泥经过碱性预处理，显示出比其高的酶活性。基材无需预处理即可使用。使用Plackett-Burman设计研究了培养基成分的作用和意义。使用MINITAB 15软件对实验性木聚糖酶活性进行统计分析。发现蛋白one，尿素，CaCl2·2H2O，吐温80，α（NH4）2.SO4，αMgSO4·7H2O3和αMnSO4′显着影响木聚糖酶的产生（8.63U / g干底物）。该酶在pH 5（8.75 U / g干燥底物），45°C（8.66 U / g干燥底物）和80％初始水分含量（8.84 U / g干燥底物）下显示最大活性，作用时间为7为了最大程度地生产酶，必须经过几天的孵育（8.57 U / g干燥底物）。在这些优化的物理条件和优化的介质中？接种harzianum？MTCC 4358并每12小时（持续8天）进行超声处理，从而产生了9.27 U / g干燥底物的更高酶促活性。

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