首页> 外文期刊>African Journal of Biotechnology >Studies on some active components and antimicrobial activities of the fermentation broth of endophytic fungi DZY16 Isolated from Eucommia ulmoides Oliv.
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Studies on some active components and antimicrobial activities of the fermentation broth of endophytic fungi DZY16 Isolated from Eucommia ulmoides Oliv.

机译:杜仲杜仲内生真菌DZY16发酵液的一些活性成分和抑菌活性的研究。

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Research into plant-derived endophytic fungi has grown in recent decades. Endophytic fungi still have enormous potential to inspire and influence modern agriculture.?In this study, the endophytic fungi DZY16 isolated from?Eucommia ulmoides?Oliv. was?tested for its bioactive components and?antimicrobial activities using?phenol-sulfuric acid method, high performance liquid chromatography method and growth inhibition measurements. The?results show?that variation trend of extracellular polysaccharide content at different growth stages of the strain DZY16 and the maximum content of extracellular polysaccharide was 2.02 g/L at the sixth day.Moreover, the fermentation broth of the?DZY16 contained guanosine, uridine and adenosine; the contents were 1.54 mg/g, 1.07 mg/g and 1.36 mg/g respectively. On the other hand, the strongest antimicrobial activity was exhibited by the acetylacetate extract of strain DZY16 against?Rhizoctonia solani?and?Gibberella zeae, showing 59.84 and 70.86% respectively.?The strain?DZY16?was identified by?internal transcribed spacer?(ITS) sequence as belonging to?Nigrospora.?The results indicate that the endophytic fungi?DZY16?of the plant?E. ulmoides?Oliv.?is a promising source of novel bioactive compounds.
机译:近几十年来,对植物来源的内生真菌的研究不断增长。内生真菌仍具有巨大的潜力,可以激发和影响现代农业。在本研究中,从杜仲杜鹃中分离出内生真菌DZY16。用苯酚-硫酸法,高效液相色谱法和生长抑制法测试了其生物活性成分和抗菌活性。结果表明,DZY16菌株在不同生长阶段的胞外多糖含量变化趋势,第六天胞外多糖最大含量为2.02 g / L。此外,DZY16菌株的发酵液中含有鸟苷,尿苷。和腺苷;含量分别为1.54mg / g,1.07mg / g和1.36mg / g。另一方面,菌株DZY16的乙酰乙酸盐提取物表现出最强的抗枯萎病菌和玉米赤霉菌的抗微生物活性,分别显示出59.84和70.86%。结果表明该植物的内生真菌为“ DZY16”。 ulmoides?Oliv。?是新型生物活性化合物的有希望的来源。

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