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Characterization of Medicago populations under cold acclimation by morphological traits and microsatellite (SSR) markers

机译:利用形态特征和微卫星(SSR)标记对冷驯化下的紫花苜蓿种群进行表征

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The study was carried on 16 accessions of annual Medicago species (M. truncatula Gaertn. M. ciliaris Krocker., M. aculeata Wild. and M. polymorpha L.). Seedlings of different accessions collected from sites of contrasting altitudes (10 to 1170 m) were subjected to different durations of low temperature regimes. Root to shoot ratios of acclimated and non acclimated plants were compared. Among the 16 accessions studied, 12 were used to assess the degree of genetic polymorphism by SSR microsatellites. Results show that accessions that originated from high altitude had a better root to shoot ratios and so had better ability to cold acclimation than accessions that originated from low altitude (lower ability to cold acclimation). Tests differentiation between species by fisher pair indicates that all species were different from each other. Results show the highest level of homozygosity for all species (> 80 %). Moreover, there were differences between populations of the same species of cold acclimation, which will encourage for a study of association between cold acclimation and molecular polymorphism.
机译:该研究对一年生紫花苜蓿物种(M. truncatula Gaertn。M. ciliaris Krocker。,M。aculeata Wild。和M. polymorpha L.)进行了研究。从海拔高度不同(10至1170 m)的地点收集的不同种质的幼苗经历了不同的低温持续时间。比较了驯化和未驯化的植物的根冠比。在研究的16个种质中,有12个用于评估SSR微卫星的遗传多态性程度。结果表明,与低海拔起源的种质相比,高海拔的种质具有更好的根冠比,因此具有更好的抗寒能力(低温适应性较低)。渔民对物种之间的差异测试表明,所有物种都互不相同。结果显示所有物种的纯合子水平最高(> 80%)。而且,相同物种的冷驯化种群之间存在差异,这将鼓励研究冷驯化与分子多态性之间的关联。

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