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首页> 外文期刊>African Journal of Biotechnology >Genetic diversity in Nigerian brinjal eggplant (Solanum melongena L.) as revealed by random amplified polymorphic DNA (RAPD) markers
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Genetic diversity in Nigerian brinjal eggplant (Solanum melongena L.) as revealed by random amplified polymorphic DNA (RAPD) markers

机译：随机扩增的多态DNA（RAPD）标记揭示了尼日利亚茄子茄子（Solanum melongena L.）的遗传多样性

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摘要

The taxonomy of Solanum melongena L., also known as brinjal eggplant, has remained difficult because previous studies to establish genetic relationships among taxa are mainly based on morphological features, which are insufficient to establish genetic affinities. In the present investigation, five highly polymorphic random amplified polymorphic DNA primers were used to describe the genetic similarity and diversity among its accessions in Nigeria. The results show a high level of polymorphism based on the banding patterns among the samples. This indicated a wide and diverse genetic base. Four distinct clusters were equally noticeable at a coefficient of 0.80 from the dendrogram generated. Generally, the grouping pattern clearly indicates that irrespective of variations in fruit traits among samples, they were still grouped together in different clusters with a high similarity coefficient value. This probably showed some genetic relatedness/closeness among the samples concerned. The study also revealed that there is no association between RAPD pattern and the geographic origin of accessions. These agreed with previously published data on the characterization of eggplant. The study disclosed that molecular methods coupled with morphological analyses could make proper classification of S. melongena and other Solanum species in Nigeria possible to achieve.
机译：Solanum melongena L.（也称为茄子茄子）的分类法仍然很困难，因为先前建立分类群之间遗传关系的研究主要基于形态特征，而这些特征不足以建立遗传亲和力。在本研究中，使用五个高度多态性随机扩增的多态性DNA引物来描述其在尼日利亚的种质之间的遗传相似性和多样性。结果显示出基于样品之间的条带模式的高水平多态性。这表明其广泛的遗传基础。从生成的树状图得出的系数为0.80时，四个不同的簇同样值得注意。通常，分组模式清楚地表明，不管样本之间的果实性状如何变化，它们仍然以相似系数值较高的不同聚类分组在一起。这可能表明相关样品之间存在一定的遗传相关性/亲密性。研究还表明，RAPD模式与种质的地理起源之间没有关联。这些与以前发表的有关茄子特性的数据相吻合。该研究表明，分子方法与形态分析相结合可以使尼日利亚的茄子和其他茄属物种进行正确分类。

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