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首页> 外文期刊>African Journal of Biotechnology >Salt-induced osmotic stress for lipid overproduction in batch culture of Chlorella vulgaris
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Salt-induced osmotic stress for lipid overproduction in batch culture of Chlorella vulgaris

机译：盐诱导的渗透压促进小球藻分批培养中脂质的过量生产

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Effect of?NaCl-induced osmotic stress?on lipid production was investigated in batch culture of?Chlorella vulgaris. Based on the facts that NaCl stress improved lipid production but inhibited cells growth at the same times, the novel?strategies of multiple?osmotic?stresses with different?NaCl?additions?(2 g/L at 80 h, 4 g/L at 100 h, and 6 g/L at 120 h) were adopted for lipid overproduction. Results show that after 180 h cultivation, lipid yield reached 3.16 g/L and intracellular lipid content was 58.6%, increased by 21.1 and 22.9%, respectively, compared to the control. Further applying the strategies to 5 L fermentor, lipid yield of 3.81 g/L was achieved at 180 h, which was 30.1% higher than the control, suggesting?application of osmotic stress?to lipid overproduction as being feasible.
机译：在寻常小球藻的分批培养中研究了NaCl诱导的渗透压对脂质产生的影响。根据NaCl胁迫可提高脂质产量但同时抑制细胞生长的事实，具有不同NaCl添加量的多重渗透胁迫的新策略（80 h为2 g / L，4 h为4 g / L）。 100小时和120小时6g / L）用于脂质过量产生。结果显示，培养180 h后，脂质产量达到3.16 g / L，细胞内脂质含量为58.6％，分别比对照提高21.1％和22.9％。将该策略进一步应用于5 L发酵罐，在180 h时脂质产量达到3.81 g / L，比对照高30.1％，这表明将渗透压应用于脂质过高生产是可行的。

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