• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Different apoptotic responses to Plasmodium chabaudi malaria in spleen and liver
【24h】

Different apoptotic responses to Plasmodium chabaudi malaria in spleen and liver

机译：脾脏和肝脏中对Chabaudi疟原虫疟疾的不同凋亡反应

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this study is to determine whether the apoptotic responses toPlasmodium chabaudi?malaria in spleen and liver via mRNA expression of three genes involved in apoptosis (Bax, Bcl-2 and Caspase-3) are similar or not and to detect if these genes could be a good marker for apoptosis due to infection with?P. chabaudi?of female C57BL/6 mice. Mice were injected intraperitoneally (ip) with 106P. chabaudi-infected erythrocytes and then scarified at days (0, 1, 4 and 8, respectively). Quantitative real time-polymerase chain reaction (PCR) was used to quantify apoptotic genes. The levels of Bax and caspase-3 were significantly increased only at days 1 and 8 in the liver cells and at day 8 in the spleen when compared with day 0. The level of B-cell lymphoma 2 (Bcl-2) was significantly increased at all three days after infection in the liver cells, but significantly decreased at all three days after infection in the spleen. In conclusion, present data has shown that infection with?P. chabaudi?stimulated apoptotic genes in the liver and spleen cells in different ways. Based on the obtained data, it can be hypothesized that the out come of malaria triggered different apoptotic pathways in the liver and spleen. Also, these apoptotic genes can be used as a reliable apoptosis detection method.?Key word:?Apoptosis, malaria, mice, liver, spleen.
机译：这项研究的目的是确定通过凋亡相关的三个基因（Bax，Bcl-2和Caspase-3）的mRNA表达，对脾脏和肝脏中的沙巴氏梭菌疟疾的凋亡反应是否相似，并检测这些基因可能是由？P感染引起的凋亡的良好标志。 Chabaudi？雌性C57BL / 6小鼠。给小鼠腹膜内（ip）注射106P。 Chabaudi感染的红细胞，然后在第几天（分别为0、1、4和8）被清除。实时定量聚合酶链反应（PCR）用于定量凋亡基因。与第0天相比，仅在肝细胞的第1天和第8天以及脾脏的第8天，Bax和caspase-3的水平才显着升高。B细胞淋巴瘤2（Bcl-2）的水平显着升高。在肝细胞感染后的所有三天都没有，但在脾脏感染后的所有三天都显着下降。总之，目前的数据表明感染了？P。 chabaudi会以不同方式刺激肝脏和脾细胞中的凋亡基因。根据获得的数据，可以假设疟疾的发作触发了肝和脾中不同的凋亡途径。而且，这些凋亡基因可以用作可靠的凋亡检测方法。关键词：凋亡，疟疾，小鼠，肝脏，脾脏。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号