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首页> 外文期刊>African Journal of Biotechnology >Serum protein fingerprint of patients with gastric cancer by SELDI technology
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Serum protein fingerprint of patients with gastric cancer by SELDI technology

机译：SELDI技术检测胃癌患者血清蛋白指纹图谱

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To study the serum protein fingerprint of patients with gastric cancer and to screen for protein molecules closely related to gastric cancer during the onset and progression of the disease using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Serum samples from 80 gastric cancers and 80 healthy volunteers. WCX2 protein chip and PBSII-C protein chips reader (Ciphergen Biosystems Ins.) were used. The protein fingerprint expression of all the serum samples and the resulting profiles between cancer and normal were analyzed with Biomarker Wizard system. A group of proteomic peaks were detected. Four differently expressed potential biomarkers were identified with the relative molecular weights of 5907, 8678, 11673 and 13725 Da. Among them, two proteins with m/z 8678 and 13725 Da down-regulated, and two proteins with m/z 5907 and 11673 Da were up-regulated in gastric cancers. This diagnostic model can distinguish gastric cancer from healthy controls with a sensitivity of 96% and a specificity of 93.3%. SELDI technology can be used to screen significant proteins of differential expression in the serum of gastric cancer patients. These different proteins could be specific biomarkers of the patients with gastric cancer in the serum and have the potential value of further investigation.
机译：使用表面增强激光解吸和电离飞行时间质谱（SELDI-TOF-S）研究胃癌患者的血清蛋白指纹图谱，并在疾病的发作和发展过程中筛选与胃癌密切相关的蛋白分子。多发性硬化症）。来自80位胃癌和80位健康志愿者的血清样本。使用了WCX2蛋白芯片和PBSII-C蛋白芯片读取器（Ciphergen Biosystems Ins。）。使用Biomarker向导系统分析所有血清样品的蛋白质指纹表达以及由此产生的癌症和正常人之间的特征。检测到一组蛋白质组峰。鉴定了四个不同表达的潜在生物标记，其相对分子量为5907、8768、11673和13725 Da。其中，m / z 8678和13725 Da的两种蛋白在胃癌中下调，而m / z 5907和11673 Da的两种蛋白在胃癌中上调。该诊断模型可将胃癌与健康对照区分开，灵敏度为96％，特异性为93.3％。 SELDI技术可用于筛选胃癌患者血清中差异表达的重要蛋白质。这些不同的蛋白质可能是胃癌患者血清中的特异性生物标志物，具有进一步研究的潜在价值。

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