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首页> 外文期刊>African Journal of Biotechnology >Identification of RAPD marker for the White Backed Plant Hopper (WBPH) resistant gene in rice
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Identification of RAPD marker for the White Backed Plant Hopper (WBPH) resistant gene in rice

机译：水稻白背稻草（WBPH）抗性基因的RAPD标记的鉴定

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The experimental material consisted of two parents Gurjari (white backed plant hopper resistant) and Jaya (white backed plant hopper susceptible) and their F2 progeny.?The purpose of the study was the identification of RAPD (Random Amplified Polymorphic DNA) marker for white backed plant hopper (WBPH) resistant gene. The RAPD analysis was done group wise as well as combined for the bulk segregant analysis (BSA). For the BSA, of the total 50 random primers surveyed, a single linked primer, OPA 08, was identified. This primer generated 8-bands, one of which, OPA08-7, was putatively linked to resistant gene as was evident by its presence in almost all the resistant bulks and vice-versa. This band had molecular weight equal to 1219.38 bp and was found in resistant parent, Gurjari, and in almost all the resistant bulks (the four susceptible bulks revealed absence of the same band) indicating the band OPA08-7 as a marker for WBPH resistance among the screened rice genotypes.
机译：实验材料由两个亲本Gurjari（抗白背飞虱）和Jaya（易受白背飞虱）及其F2后代组成。本研究的目的是鉴定白背白兔的RAPD（随机扩增多态性DNA）标记。植物跳跃者（WBPH）抗性基因。 RAPD分析是按组进行的，也可以进行整体隔离分析（BSA）的组合。对于BSA，在调查的总共50个随机引物中，鉴定了一个单连接引物OPA 08。该引物产生了8条带，其中一条，即OPA08-7，被推定与抗性基因相连，这一点在几乎所有抗性体中都存在，反之亦然。该条带的分子量等于1219.38 bp，存在于抗性亲本Gurjari和几乎所有抗性块体中（四个易感块体均显示相同的条带），表明OPA08-7条带是WBPH抗性的标记筛选的水稻基因型。

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