首页> 外文期刊>African Journal of Biotechnology >Assessment of an oil palm population from Nigerian Institute for Oil Palm Research (NIFOR) for simple sequence repeat (SSR) marker application
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Assessment of an oil palm population from Nigerian Institute for Oil Palm Research (NIFOR) for simple sequence repeat (SSR) marker application

机译:对尼日利亚油棕研究所(NIFOR)的油棕种群进行评估,以进行简单序列重复(SSR)标记应用

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Oil palm (Elaeis guineensis Jacq.), a monocotyledonous plant belonging to the Arecaceae family, is one of the most important oil crops in the world. In Nigeria, oil palm has benefited immensely from conventional breeding efforts resulting in high yields that have been achieved with this breeding material. However, oil palm breeding is slow and time-consuming due to a breeding cycle of about 10 years. In addition, the process of outcrossing leads to high variation in yield components and vegetative traits. Although DNA marker technologies offer great possibilities for plant breeding through marker-assisted selection, there are so far no reports of its application to oil palm breeding in Nigeria. In this study, 32 SSR markers were used for the assessment of marker application in an oil palm breeding population coming from the extensive breeding program at the Nigerian Institute for Oil Palm Research (NIFOR). Seven SSR markers out of the 32 tested (22%) segregated in the progeny 12 (tenera x Deli dura). SSR markers mEgCIR0059, mEgCIR1917, mEgCIR3260, mEgCIR3275, mEgCIR3533 and mEgCIR3557 proved to be fully informative markers following a segregation ratio of 1:1:1:1, while marker mEgCIR0074 segregated in a 1:1 ratio.?Key?words: Oil palm, microsatellite marker, marker-assisted selection, NIFOR.
机译:油棕(Elaeis guineensis Jacq。)是属于槟榔科的单子叶植物,是世界上最重要的油料作物之一。在尼日利亚,油棕得益于常规育种工作,从而使这种育种材料实现了高产。但是,由于约10年的繁殖周期,油棕的繁殖缓慢且耗时。另外,异源杂交的过程导致产量成分和营养性状的高度变化。尽管DNA标记技术为通过标记辅助选择的植物育种提供了巨大的可能性,但迄今为止,尚无关于将其应用于尼日利亚油棕育种的报道。在这项研究中,使用32个SSR标记对来自尼日利亚油棕研究所(NIFOR)广泛育种计划的油棕育种种群中的标记应用进行评估。在后代12(tenera x Deli dura)中分离的32个测试个体中有7个SSR标记(22%)。 SSR标记mEgCIR0059,mEgCIR1917,mEgCIR3260,mEgCIR3275,mEgCIR3533和mEgCIR3557在分离比为1:1:1:1的情况下被证明是完全有用的标记,而标记mEgCIR0074以1:1的比例分离。 ,微卫星标记,标记辅助选择,NIFOR。

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