首页> 外文期刊>Journal of Agricultural Science >Genetic Relationships between Elite Oil Palms from Nigeria and Selected Breeding and Germplasm Materials from Malaysia via Simple Sequence Repeat (SSR) Markers
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Genetic Relationships between Elite Oil Palms from Nigeria and Selected Breeding and Germplasm Materials from Malaysia via Simple Sequence Repeat (SSR) Markers

机译:通过简单序列重复(SSR)标记,尼日利亚的优良油棕与马来西亚的某些选育和种质材料之间的遗传关系

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Nine SSR markers were used to study the genetic relationships among 26 elite oil palm materials from Nigeria and Malaysia. The Nigerian elite materials comprised 15 Nigerian Institute for Oil Palm Research (NIFOR) oil palm parental genotypes. The 11 Malaysian genotypes included 2 dura and 2 pisifera advanced breeding lines, 3 palms from natural populations of Nigeria, and 2 from each of the Angola, and Madagascar natural oil palm collections maintained at the Malaysian Palm Oil Board (MPOB). The results revealed a high percentage of polymorphic loci (83.3%) in the entire materials with average polymorphic information content (PIC) of 0.7325. Two SSR markers showed relatively high PIC namely sMg00016 and sMg00179 (0.8018 and 0.8509, respectively). The mean number of alleles varied from 1.333 in Madagascar germplasm material to 4.889 in NIFOR tenera materials (mean = 2.958). The observed heterozygosity varied from 0.167 in Madagascar material to 0.778 in Nigeria germplasm materials (mean = 0.575) and the expected heterozygosity from 0.153 to 0.643. The various oil palm provenances showed significant genetic differentiation (FST = 0.177, P = 0.001) indicating the existence of a genetic structure among the materials used. UPGMA analysis based on Rogers’ dissimilarity coefficient matrix displayed two main clusters, one separating Madagascar accessions from the rest. PCoA showed that the NIFOR breeding parents clustered closely with MPOB’s Nigeria and Angola-derived materials suggesting a common origin of mainland genotypes. The high genetic diversity observed among the MPOB’s germplasm materials highlights the need for proper characterization of entire NIFOR oil palm germplasm with SSR markers to facilitate effective utilization in the breeding programme.
机译:使用九个SSR标记研究了来自尼日利亚和马来西亚的26种精英油棕材料之间的遗传关系。尼日利亚的精英材料包括15个尼日利亚油棕研究所(NIFOR)油棕亲本基因型。马来西亚的11种基因型包括2个硬脑膜和2个pisifera先进育种系,尼日利亚自然种群中的3棵棕榈树和安哥拉各州的2种棕榈树,以及由马来西亚棕榈油委员会(MPOB)保存的马达加斯加天然油棕榈树。结果显示,在所有材料中,多态位点的比例很高(83.3%),平均多态信息含量(PIC)为0.7325。两个SSR标记显示出相对较高的PIC,即sMg00016和sMg00179(分别为0.8018和0.8509)。等位基因的平均数从马达加斯加种质材料中的1.333变化到NIFOR特里拉材料中的4.889(平均值= 2.958)。观察到的杂合度在马达加斯加物质中为0.167,在尼日利亚种质物质中为0.778(平均值= 0.575),预期杂合度在0.153至0.643之间。各种油棕种源显示出显着的遗传分化(FST = 0.177,P = 0.001),表明所用材料之间存在遗传结构。根据罗杰斯的相异系数矩阵进行的UPGMA分析显示出两个主要类,其中一个将马达加斯加种与其他种分开。 PCoA表明,NIFOR的育种亲本与MPOB的尼日利亚和安哥拉来源的材料密切相关,表明大陆基因型是共同的起源。在MPOB种质材料中观察到的高度遗传多样性表明,需要正确鉴定带有SSR标记的整个NIFOR油棕种质,以促进在育种计划中的有效利用。

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