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首页> 外文期刊>African Journal of Biotechnology >Identifying salt stress-responsive transcripts from Roselle (Hibiscus sabdariffa L.) roots by differential display
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Identifying salt stress-responsive transcripts from Roselle (Hibiscus sabdariffa L.) roots by differential display

机译：通过差异显示鉴定玫瑰茄（Hiscus sabdariffa L.）根的盐胁迫响应性转录本

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No previous study has been reported on the salt-modulated gene(s) of roselle (Hibiscus sabdariffa L.). Identifying the potentially novel transcripts responsible for salt stress tolerance in roselle will increase knowledge of the molecular mechanism underlying salt stress responses. In this study, differential display reverse transcriptase PCR (DDRT-PCR) was used to compare the overall differences in gene expression between salt-stressed and control plants. A total of 81 primer combinations were used and false positive clones were rejected during a screening and quality control assay. The remaining nine cDNA transcript fragments were extracted from the gel, reamplified, cloned and sequenced. A homology search revealed that four transcripts showed significant homology with known genes. Out of five transcripts, real-time PCR demonstrated that four exhibited high expression in salt-stressed root tissues relative to the control and one transcript was down-regulated. These transcripts may be useful for improving tolerance in salt stress-sensitive plants.
机译：以前没有关于玫瑰茄（Hibiscus sabdariffa L.）的盐调节基因的研究的报道。在玫瑰茄中鉴定出负责盐胁迫耐受性的潜在新转录本，将增加对盐胁迫响应的分子机制的了解。在这项研究中，差异显示逆转录酶PCR（DDRT-PCR）用于比较盐胁迫和对照植物之间基因表达的总体差异。在筛选和质量控制测定过程中，总共使用了81种引物组合，并剔除了假阳性克隆。从凝胶中提取剩余的9个cDNA转录片段，再扩增，克隆并测序。同源性搜索显示，四个转录本与已知基因显示出显着同源性。在五种转录本中，实时PCR证实相对于对照，四种在盐胁迫的根组织中表现出高表达，而一种转录本被下调。这些转录本可用于提高盐胁迫敏感性植物的耐受性。

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