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首页> 外文期刊>African Journal of Biotechnology >In vitro multiple shoot bud induction and regeneration from plumule junction explants of pigeon pea [Cajanus cajan (L.) Mill sp.] cultivars
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In vitro multiple shoot bud induction and regeneration from plumule junction explants of pigeon pea [Cajanus cajan (L.) Mill sp.] cultivars

机译：木豆[Cajanus cajan（L.）Mill sp。]品种的羽状结外植体的体外多芽萌芽诱导和再生

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摘要

The response of eleven Indian cultivars of pigeon pea for in vitro multiple shoot bud induction and regeneration from plumule junction explants under variable concentration of 6-benzyl amino purine (BAP), kinetin and thiadiazuron (TDZ) was assessed in the present study. The cultivar IPA-3088 showed best response with a maximum of 20 buds per explants in Murashige and Skoog (MS) media supplemented with 0.05 mgL-1 TDZ. Among the hormones tested, lower concentration of TDZ gave the best response for these cultivars though higher concentration of BAP was also effective in multiple shoot bud induction and regeneration from plumule junction explants. The elongation of multiple shoot buds was achieved in the same medium and the nature of the regenerants in most of the cases was shoots, though in few cases shoot buds and shoot primoridia were also observed. Rooting of plumule junction derived shootlets was found to be better in the presence of NAA as compared to IAA and IBA for most of the cultivars producing maximum number of primary roots. The best responding cultivar IPA 3088 showed efficient rooting in the presence of 0.2 mgL-1 of NAA. The regenerated plantlets were acclimatized in soil with percentage of acclimatization varying from 40-80% for different cultivars.
机译：在本研究中，评估了11种印度豌豆木豆在可变浓度的6-苄基氨基嘌呤（BAP），激动素和噻二氮酮（TDZ）下对多囊芽外植体诱导的多芽萌芽和再生的响应。 IPA-3088品种在补充有0.05 mgL-1 TDZ的Murashige和Skoog（MS）培养基中显示出最佳的响应，每个外植体最多有20个芽。在所测试的激素中，较低的TDZ浓度对这些品种产生最佳响应，尽管较高的BAP浓度也能有效诱导多芽萌芽和胚芽外植体再生。在相同的培养基中可以实现多个芽的伸长，并且在大多数情况下，再生物的性质是芽，尽管在少数情况下也观察到芽和原基。对于大多数产生最大根数的品种，发现在存在NAA的条件下，与IAA和IBA相比，羽状结衍生的小芽的生根更好。响应最佳的品种IPA 3088在存在0.2 mgL-1的NAA时显示出有效的生根。再生的幼苗在土壤中适应，不同品种的适应百分比在40％至80％之间。

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