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首页> 外文期刊>International Journal of Applied Biology and Pharmaceutical Technology >EFFICIENT SHOOT INDUCTION AND PLANT REGENERATION OF PIGEON PEA [ CAJANUS CAJAN (L.) MILLISP.] ICPL 87 VARIETY USING LEAF PETIOLE EXPLANTS
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EFFICIENT SHOOT INDUCTION AND PLANT REGENERATION OF PIGEON PEA [ CAJANUS CAJAN (L.) MILLISP.] ICPL 87 VARIETY USING LEAF PETIOLE EXPLANTS

机译：豌豆豌豆[CAJANUS CAJAN（L.）MILLISP。] ICPL 87的有效枝叶诱导和植株再生

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Pigeon pea or red gram is a high protein legume crop of semi-arid tropics and sub-tropics, which ranks sixth in production among the other legumes. It is used as a major source of protein (21%) in many countries like India. In the present study, an efficient in vitro regeneration protocol was developed for ICPL 87 variety of pigeon pea ( Cajanus cajan L.) using leaf petiole explants. Leaf petiole explants of six day old seedlings were cultured on MS media supplemented with different concentrations of 6-Benzylaminopurine and α-napthaleneacetic acid for multiple shoot bud induction. MS media supplemented with 0.5 mg/l α-napthalene acetic acid + 2 mg/l 6-Benzylaminopurine hormonal concentrations induced highest number of shoot buds compared to other combinations. Induced multiple shoots were elongated on MS media fortified with 6-Benzylaminopurine, α-napthaleneacetic acid and Gibberellic acid hormonal combinations. Maximum number of shoots were elongated with hormonal combination of 1 mg/l 6-Benzylaminopurine + 0.2 mg/l α-napthaleneacetic acid + 3 mg/l Gibberellic acid. These well-elongated shoots were rooted on MS media enriched with different concentrations of Indole-3-butyric acid and maximum percentage of rooting was observed on MS media supplemented with 0.8 mg/l Indole-3-butyric acid concentration. Finally, these rooted plants were transferred to soil and vermiculate mixture in 1:1 ratio for hardening and acclimatization. The protocol standardized can be used for genetic transformation of pigeon pea to generate transgenic pigeon pea plants
机译：木豆或红克是半干旱热带和亚热带的一种高蛋白豆类作物，在其他豆类中居第六位。在印度等许多国家/地区，它被用作蛋白质的主要来源（占21％）。在本研究中，使用叶柄外植体为ICPL 87木豆（Cajanus cajan L.）开发了一种有效的体外再生方案。在补充了不同浓度的6-苄基氨基嘌呤和α-萘乙酸的MS培养基上培养六日龄幼苗的叶柄外植体，以诱导多发芽。与其他组合相比，补充有0.5 mg / lα-萘萘乙​​酸+ 2 mg / l 6-苄基氨基嘌呤激素浓度的MS培养基诱导出最高的芽芽数。在用6-苄基氨基嘌呤，α-萘乙酸和赤霉素激素组合强化的MS培养基上将诱导的多次芽延长。用1 mg / l的6-苄基氨基嘌呤+ 0.2 mg / l的α-萘乙酸+3 mg / l的赤霉素的激素组合延长最大芽数。这些充分伸长的芽生根于富含不同浓度的吲哚-3-丁酸的MS培养基上，并且在添加0.8 mg / l吲哚-3-丁酸浓度的MS培养基上观察到最大的生根百分比。最后，将这些生根的植物转移到土壤中，并按1：1的比例混合混合物，以使其硬化和适应环境。标准化的协议可用于木豆的遗传转化以产生转基因木豆植物

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