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AN EFFICIENT REGENERATION SYSTEM VIA DIRECT SOMATIC EMBRYOGENESIS IN TURMERIC (CURCUMA LONGA L.) USING LEAF SHEATH EXPLANTS
AN EFFICIENT REGENERATION SYSTEM VIA DIRECT SOMATIC EMBRYOGENESIS IN TURMERIC (CURCUMA LONGA L.) USING LEAF SHEATH EXPLANTS
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机译：使用叶鞘的方法通过涡轮（CURCUMA LONGA L.）直接体细胞胚发生的高效再生系统
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摘要
This study describes for the first time a simple and quick direct somatic embryogenesis and plant regeneration protocol for turmeric {Curcuma longa L.) using leaf base explants. Preincubation of explants was carried out on solid MS medium containing 4.49 uM 2,4-dichlorophenoxy acetic acid (2,4-D) for 20 days under continuous dark condition. Subsequently transfer of the explants for two weeks to MS liquid medium containing 1.32 μM 6-benzyladenine (BA) under illuminated condition resulted in the highest percentage of somatic embryo (91.1 %) induction. Matured somatic embryos germinated readily and the maximal plantlet development (87.0 %) was achieved on 1/2-strength MS medium containing gibberellic acid (GA3) under dark condition. Histological analysis revealed that the proembryogenic masses were initiated directly from the area near the vascular tissues of the base explants Presence of clear protoderm and procambial strands confirmed that these structures were true somatic embryos. Those somatic embryo derived plantlets were successfully acclimatized ex vitro and transplanted to pots.
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机译：这项研究首次描述了使用叶基外植体对姜黄（Curcuma longa L.）进行简单，快速的直接体细胞胚发生和植物再生的方法。外植体的预培养在连续黑暗条件下于含有4.49 uM 2,4-二氯苯氧基乙酸（2,4-D）的固体MS培养基上进行20天。随后将外植体在光照条件下转移到含有1.32μM6-苄基腺嘌呤（BA）的MS液体培养基中两周，导致诱导的体细胞胚百分比最高（91.1％）。成熟的体细胞胚容易发芽，并且在黑暗条件下，在含有赤霉素（GA3）的1/2强度MS培养基上达到了最大的幼苗发育（87.0％）。组织学分析表明，胚原性肿块是直接从基本外植体维管组织附近的区域开始的。存在清晰的原皮和变形层，证实这些结构是真正的体细胞胚。这些体细胞胚衍生的小植株已成功地进行了体外驯化，并移植到盆中。
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