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首页> 外文期刊>African Journal of Biotechnology >Inducing and identifying artificially-induced polyploidy in bananas
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Inducing and identifying artificially-induced polyploidy in bananas

机译：诱导和鉴定香蕉中的人工诱导的多倍体

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摘要

In the present work, polyploidy was induced in the diploid banana varieties 'Malbut', 'Gold', 'Lidi', and 'Thong Dok Mak' through the use of colchicine and oryzalin, and that condition was identified through stomatal analysis, flow cytometry, and chromosome counts. Shoots produced in vitro were treated with colchicine at concentrations of 0, 2.5, 7.5 and 12.5 mM for 24 and 48 h, and with oryzalin at 0, 10, 30 and 50 mM for 4 and 7 days. Young leaves were scanned by electron microscopy to determine their stomatal areas (polar diameter × equatorial diameter) and numbers for polyploid identification by stomatal analysis. Polyploid identification by way of flow cytometry analysis used samples of young leaves that were crushed to release their nuclei, with subsequent staining with propidium iodide; ten thousand nuclei were analyzed for each sample. For cytogenetic analyses, root tips were pretreated with 0.002 M 8-HQ for 3 h, fixed in Carnoy solution for 24 h, subjected to conventional squashing techniques, and stained with 10% Giemsa. We identified four tetraploid plants and six mixoploids using these three identification techniques.
机译：在目前的工作中，通过使用秋水仙碱和米扎林，在二倍体香蕉品种“ Malbut”，“ Gold”，“ Lidi”和“ Thong Dok Mak”中诱导了多倍体，并通过气孔分析，流式细胞术确定了该病状。和染色体计数。用浓度分别为0、2.5、7.5和12.5 mM的秋水仙碱处理24和48小时，并用0、10、30和50 mM的谷氨酰胺处理4到7天的体外芽。通过电子显微镜扫描幼叶，以确定其气孔面积（极径×赤道直径）和通过气孔分析鉴定多倍体的数量。通过流式细胞术分析鉴定多倍体，使用的是嫩叶样品，将其压碎以释放其细胞核，然后用碘化丙啶染色。每个样品分析了10,000个核。对于细胞遗传学分析，将根尖用0.002 M 8-HQ预处理3 h​​，在Carnoy溶液中固定24 h，进行常规压榨技术，并用10％Giemsa染色。我们使用这三种鉴定技术鉴定了4种四倍体植物和6种混合倍体。

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