• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Optimization of embryogenic-callus induction and embryogenesis of Glycyrrhiza glabra
【24h】

Optimization of embryogenic-callus induction and embryogenesis of Glycyrrhiza glabra

机译：甘草胚性愈伤组织诱导和胚发生的优化

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glabridin is a major?biologically active flavonoid isolated specifically from the root ofGlycyrrhiza?glabra, which has many pharmacological activities.?The production of the wild?G. glabra?was sharply decreased due to immoderate and ruinous utilization.?In vitro?regeneration via somatic embryogenesis is important for clonal propagation and genetic transformation. In this paper, factors affecting the embryogenic calli and embryo induction, maintenance and multiplication of?G. glabra?are assessed. The results showed that?the explants of hypocotyl give the highest calli formation frequency of 93.3%?on Murashige and Skoog (MS) medium containing 2.0 mg/L 6-benzylaminopurine (6-BA) and 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D).?The maximum efficiency of embryo were obtained on MS medium with 0.5 mg/L 6-BA + 0.5 mg/L??kinetin zeatin (KT) + 0.1 mg/L?indole-3-butyric acid (IBA); the embryos could develop further on medium with 1000 mg/L malt extract (ME). The occurrence of the embryogenic calli andproglobular?embryo were studied by?histological section, indicating the single cell origin of the embryogensis of?G. glabra.?With the protocol reported herein, some green embryo-like cultures were obtained, from which shoots were successfully regenerated in the germinated medium?after 10 months of subculture.
机译：Glabridin是一种主要的具有生物活性的类黄酮，特别是从甘草的根中分离出来的，具有许多药理活性。由于不适当和破坏性的利用，glabra急剧减少。通过体细胞胚发生的体外再生对于克隆繁殖和遗传转化很重要。本文探讨了影响胚性愈伤组织和胚胎诱导，维持和增殖的因素。 glabra？被评估。结果表明，在含有2.0 mg / L 6-苄氨基嘌呤（6-BA）和0.5 mg / L 2,4-二氯苯氧基乙酸的Murashige和Skoog（MS）培养基上，下胚轴外植体的愈伤组织形成频率最高，为93.3％。酸（2,4-D）。在具有0.5 mg / L 6-BA + 0.5 mg / L肌动蛋白玉米素（KT）+ 0.1 mg / L？吲哚-3-的MS培养基上获得最大的胚效率。丁酸（IBA）;胚可以在含有1000 mg / L麦芽提取物（ME）的培养基上进一步发育。通过组织学切片研究了胚性愈伤组织和原球胚的发生，表明了GG胚的单细胞起源。通过本文报道的方案，获得了一些绿色的胚样培养物，继代培养10个月后，在萌发的培养基中从中成功地再生了芽。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号