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Analysis of similarities within different date palm cultivars of Al-Ahsa oasis in Saudi Arabia

机译:沙特阿拉伯Al-Ahsa绿洲不同枣椰子品种内的相似性分析

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Date palm (Phoenix dactylifera L.) is widely cultivated in Saudi Arabia, with annual production estimated to be above one million tons. Of the 450 cultivars that are commonly used, 70 cultivars are found in the Eastern province and 20 cultivars are well known and common in the Al-Ahsa oasis. In this study, thirty seven date palm samples representing nine cultivars collected from six locations in the Al-Ahsa oasis, Saudi Arabia. Total proteins were extracted and subjected to analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed several differences within the cultivars. Overall polymorphism? ranged between 12 and 76%, with the highest being 76% within Helali cultivar, and the least being 12% within OmRohaim cultivar. These results support the conclusion that some palms are grown from seeds resembling a known cultivar, which are later distributed as the known cultivar. These results revealed differences in the degree of similarity between the cultivars from 0.059 to 0.579, estimated according to Jaccard`s similarity method based on the presence and absence of protein bands. Khosab and Shehel cultivars were closely related to each other with 0.579 degrees of similarity, both separated by phylogenetic dendrogram in mini-cluster, which have been confirmed by the 1st principal components. The mini-cluster was related to OmRohaim and Khonaizi cultivars within a larger cluster. Shishi, Garrah and Helali were related cultivars, with degrees of similarities ranging between 0.250 and 0.455, and were separated in one cluster by phylogenetic dendrogram. The close relationship between the cultivars may indicate that they originated from the same ancestor. Further molecular and biochemical studies are needed to enrich our knowledge about the relationships among? date palm cultivars.
机译:枣椰子(Phoenix dactylifera L.)在沙特阿拉伯广泛种植,估计年产量超过一百万吨。在450个常用品种中,东部省份发现了70个品种,Al-Ahsa绿洲中有20个著名品种并很常见。在这项研究中,从沙特阿拉伯Al-Ahsa绿洲的六个地点采集的代表9个品种的37个枣椰子样品。提取总蛋白并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)进行分析。结果揭示了品种内部的几个差异。总体多态性?含量介于12%至76%之间,最高的是Helali品种,为76%,最小的是OmRohaim品种,为12%。这些结果支持这样的结论,即一些棕榈是从类似于已知品种的种子中生长出来的,这些种子后来又作为已知品种分发。这些结果揭示了根据Jaccard的相似性方法基于存在和不存在蛋白质条带而估计的品种之间的相似度差异为0.059至0.579。 Khosab和Shehel品种彼此之间具有密切的相似性,相似度为0.579,二者均通过小型聚类中的系统发育树状图分开,这已由第一主要成分确认。小型集群与较大集群中的OmRohaim和Khonaizi品种有关。 Shishi,Garrah和Helali是相关品种,相似度在0.250至0.455之间,并通过系统发育树状图将其分为一类。品种之间的密切关系可能表明它们起源于同一祖先。需要进一步的分子和生化研究来丰富我们之间的关系知识。枣椰子品种。

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