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首页> 外文期刊>African Journal of Biotechnology >Evaluation of potential bio-control agents on root-knot nematode Meloidogyne incognita and wilt causing fungus Fusarium oxysporum f.sp. conglutinans in vitro
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Evaluation of potential bio-control agents on root-knot nematode Meloidogyne incognita and wilt causing fungus Fusarium oxysporum f.sp. conglutinans in vitro

机译：评价潜在的生物防治剂对根结线虫根结线虫和引起真菌枯萎的枯萎镰刀菌f.sp.体外粘连

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Indigenous strains of Trichoderma viride (ITCC No. 6889), Pseudomonas fluorescens (ITCC No. B0034) and Purpureocillium lilacinum (ITCC No.6887) were isolated from undisturbed forest eco-system of Southern India. These three bio-mediators were evaluated for their antagonism towards root knot nematode, Meloidogyne incognita and Fusarium oxysporum f.sp. conglutinans in vitro. Cell free culture filtrate of these strains significantly inhibited the egg hatching and caused juvenile (J2) mortality of M. incognita at 25, 50, 75 and 100% concentrations. Maximum inhibition in egg hatching and juvenile mortality were recorded in P. lilacinum as 94.21 and 91.28%, respectively after 120 h. It was followed by T. viride and P. fluorescens which recorded 92.72 and 91.46% and 89.12 and 90.14% inhibition in egg hatching and juvenile mortality, respectively after 120 h. Antagonism of T. viride on F. oxysporum was recorded maximum on the 5th day as 45.82%. Similarly, the antagonism on the 5th day for both the bio-agents of P. lilacinum and P. fluorescens were recorded as 45.26 and 44.19%, respectively.
机译：从印度南部未受干扰的森林生态系统中分离出木霉木霉（ITCC No. 6889），荧光假单胞菌（ITCC No. B0034）和淡紫色紫癜（ITCC No.6887）的本地菌株。评价了这三种生物介质对根结线虫，南方根结线虫和尖孢镰刀菌的拮抗作用。体外粘连。这些菌株的无细胞培养滤液显着抑制卵孵化，并在25％，50％，75％和100％的浓度下导致隐姓氏梭菌的幼年（J2）死亡率。 120小时后，紫丁香中卵孵化和幼虫死亡率的最大抑制分别为94.21和91.28％。紧随其后的是T. viride和P. fluorescens，它们分别在120小时后分别抑制了92.72％和91.46％以及89.12％和90.14％的卵孵化率和幼虫死亡率。在第5天记录到T. viride对尖孢镰刀菌的拮抗作用最大，为45.82％。同样，第5天，紫丁香假单胞菌和荧光假单胞菌的生物拮抗作用分别记录为45.26％和44.19％。

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