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首页> 外文期刊>African Journal of Biotechnology >Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer
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Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer

机译：以小黑麦为底物和诱导物来源的固态发酵优化植酸酶的生产

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This study was carried out to evaluate the process of phytase production by Aspergillus niger in solid-state fermentation (SSF) using triticale waste. A waste that currently has no use was reported for this biotechnological process, and is of high impact due to the null use. The process was carried out using an additive free medium, supplemented with only one nitrogen source. Under these conditions, phytase activity of 7.45 U/g dry substrate (DS) was obtained. The process was optimized using different additives such as dextrose, lactose, Tween 80 and potassium chloride. For fermentation maximization, two experimental designs were used: 1) Plackett-Burman design (PBD) and 2) the Box-Behnken design (BBD). PBD was used to evaluate the effect of related variables on the production of phytase, as well as their level of significance in the process, while BBD was used for optimal conditions determination. The process was conducted with Petri dishes and a maximum enzyme activity of 25.8 IU/g DS was obtained. Subsequently, SSF was carried out in a tray to increase the amount of fermented substrate and phytase activity of 23.63 IU/g DS was obtained. The results of this study suggest a minimal decrease (8.4%) in enzyme production with scaling.
机译：这项研究旨在评估黑曲霉在使用黑小麦废料的固态发酵（SSF）中产生植酸酶的过程。据报道，目前没有用于该生物技术过程的废物，由于没有使用而产生了巨大影响。该过程是使用无添加剂的介质进行的，仅添加了一种氮源。在这些条件下，植酸酶活性为7.45 U / g干底物（DS）。使用不同的添加剂（例如葡萄糖，乳糖，吐温80和氯化钾）优化了该过程。为了使发酵最大化，使用了两个实验设计：1）Plackett-Burman设计（PBD）和2）Box-Behnken设计（BBD）。 PBD用于评估相关变量对植酸酶生产的影响及其在过程中的重要性水平，而BBD用于确定最佳条件。该过程在培养皿中进行，最大酶活性为25.8 IU / g DS。随后，在托盘中进行SSF以增加发酵底物的量，并获得23.63 IU / g DS的植酸酶活性。这项研究的结果表明，随着水垢的产生，酶的产量将有最小的下降（8.4％）。

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