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首页> 外文期刊>African Journal of Biotechnology >Changes in specific activity of ascorbate peroxidase during seed development of pea (Pisum sativum L.) treated with salicylic acid
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Changes in specific activity of ascorbate peroxidase during seed development of pea (Pisum sativum L.) treated with salicylic acid

机译：水杨酸处理豌豆（Pisum sativum L.）种子发育过程中抗坏血酸过氧化物酶比活性的变化

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摘要

A field split-split plot experiment in randomized complete block design was conducted during years 2003-04 and 2004-05 to evaluate the effects of salicylic acid (SA) at concentrations of 0, 10-4?and 10-5?M on four pea varieties (Meteor, Climax, Greenfeast and Rondo). Four phenological growth stages were selected of seed and fruit development (BBCH 73, BBCH 77, BBCH 83 and BBCH 88) for enzyme assay. Aqueous SA was applied by three different modes: Seed treatment, seed treatment plus foliar spray and foliar spray.?Maximum ascorbate peroxidase (APX) activity was exhibited by the variety Rondo as compared to other three varieties during both years of study. At BBCH 77, all the varieties showed maximum specific activities of APX which gradually decreased in BBCH 83 and BBCH 88 phenological growth stages. The specific activities of APX were recorded as maximum in the pea seeds treated with SA concentration 10-4?M as compared to 10-5?M and 0 M during both years of study. In comparison of modes of application of SA, it was observed that maximum specific activity of APX was in plants which were given seed treatment plus foliar spray (STFS) as compared to only seed treatment (ST) or foliar spray (FS).
机译：在2003-04年和2004-05年间，进行了随机完整区块设计的现场分割图试验，以评估水杨酸（SA）在0、10-4？和10-5？M浓度下对四种水杨酸的影响豌豆品种（流星，高潮，Greenfeast和Rondo）。选择了种子和果实发育的四个物候生长阶段（BBCH 73，BBCH 77，BBCH 83和BBCH 88）进行酶分析。 SA的水处理方式有三种：种子处理，种子处理，叶面喷雾和叶面喷雾。在两年的研究中，Rondo与其他三个品种相比，具有最高的抗坏血酸过氧化物酶（APX）活性。在BBCH 77处，所有品种均显示出最大的APX比活度，并在BBCH 83和BBCH 88物候生长阶段逐渐降低。在两年的研究中，用SA浓度为10-4？M和0 M的豌豆种子处理的豌豆种子中，APX的比活最大。比较SA的施用方式，观察到APX的最大比活性是在进行种子处理加叶面喷雾（STFS）的植物中，与仅种子处理（ST）或叶面喷雾（FS）相比。

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