首页> 外文期刊>African Journal of Biotechnology >Induction of callogenesis in Ipomoea obscura (L.) Ker-Gawl, a little known medicinal plant
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Induction of callogenesis in Ipomoea obscura (L.) Ker-Gawl, a little known medicinal plant

机译:番石榴(Ipomoea obscura(L.)Ker-Gawl,一种鲜为人知的药用植物,其愈伤组织的诱导

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The present study was undertaken to explore the potentiality of?in vitromicropropagation (by callus induction) of?Ipomoea obscura?(Convolvulaceae), a perennial herb. This medicinal plant grows widely throughout Indian subcontinent. Axenically grown cotyledonary leaves regenerated into profuse calli, in various combinations of naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 2,4-dichlorophenoxyacetic acid (2,4 D) with benzylaminopurine (BAP). Microroots emerged from these calli when subjected to various combinations of NAA and BAP. Maximum number of root meristems and microroots were formed in 1.25 mg/L NAA with the combination of 0.5 mg/L BAP (1:4 v/v). Leaf, nodal and internodal segments as explants were cultured on MS media containing 2,4 D at 0.5, 1.25, 2.50, 5.0 and 10.0 mg/L, for callus induction. Only leaf tips initiated callogenesis with highest response at 10 mg/l 2,4 D and lowest with 0.5 mg/l 2.4 D. This is the first report of?in vitro?response of?I. obscura.
机译:本研究的目的是探索多年生草本植物番薯(Ipomoea obscura)(旋花科)的体外微繁(通过愈伤组织诱导)的潜力。这种药用植物在印度次大陆广泛生长。萘乙酸(NAA),吲哚-3-乙酸(IAA),吲哚-3-丁酸(IBA)和2,4-二氯苯氧基乙酸(2,4 D )与苄基氨基嘌呤(BAP)。当经受NAA和BAP的各种组合时,微根从这些愈伤组织中出现。 1.25 mg / L NAA和0.5 mg / L BAP(1:4 v / v)的结合形成了最大的根分生组织和微根。作为外植体的叶,节和节间节段在含有2,4 D的MS培养基上以0.5、1.25、2.50、5.0和10.0 mg / L进行培养,以诱导愈伤组织。仅叶尖开始愈伤组织,其在10 mg / l 2,4 D时响应最高,而在0.5 mg / l 2.4 D时响应最低。这是I体外应答的首次报道。晦涩。

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