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首页> 外文期刊>African Journal of Biotechnology >Preliminary biological screening of microbes isolated from cow dung in Kampar
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Preliminary biological screening of microbes isolated from cow dung in Kampar

机译：从金宝牛粪中分离出的微生物的初步生物学筛选

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Five distinct morphologically and physiologically isolates were isolated from cow dung at Kampar, Perak, Malaysia and cultured on nutrient agar (NA) plates. Morphological studies including microscopic examination, gram-staining and endospore staining were performed. Isolates K1 and K5 were gram-negative, while isolates K2, K3 and K4 were gram-positive. Isolates K2 and K4 were able to form endospore. Susceptibilities of five isolates to 17 different types of antibiotics were evaluated using the Kirby-Bauer assay. Each individual isolate was resistant to at least 35% of the antibiotics tested. Antibacterial activities against 11 types of test organisms were evaluated. Isolate K4 produced antibacterial agent which inhibited the growth of?Escherichia coli. Enzymatic assay for the presence of 22 types of enzymes were screened. All isolates produced protease, lipase and esterase lipase. Further studies will be done for identification of all the species.
机译：从马来西亚霹雳州金宝的牛粪中分离出五种不同的形态和生理分离株，并在营养琼脂（NA）平板上培养。进行了形态学研究，包括显微镜检查，革兰氏染色和孢子内染色。菌株K1和K5为革兰氏阴性，而菌株K2，K3和K4为革兰氏阳性。分离物K2和K4能够形成内生孢子。使用Kirby-Bauer分析评估了五种分离物对17种不同类型抗生素的敏感性。每个单独的分离株均对至少35％的测试抗生素具有抗药性。评估了对11种测试生物的抗菌活性。分离出的K4产生的抗菌剂抑制了大肠杆菌的生长。筛选了22种酶的存在的酶促测定。所有分离物均产生蛋白酶，脂肪酶和酯酶脂肪酶。将进行进一步的研究以鉴定所有物种。

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