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Genetic diversity and population structure of Chinese honeybees (Apis cerana) under microsatellite markers

机译:微卫星标记条件下中国蜜蜂的遗传多样性和种群结构

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Using 21 microsatellite markers and PCR method, the polymorphisms of 20?Apis cerana?honeybee populations across China was investigated and the genetic structure and diversity of the populations were explored. The results showed that 507 alleles (mean 24.14 per locus, ranging from 13 to 45) were observed in 842 honeybees.?Wuding bee?had the highest level of heterozygosity (0.695), and the lowest estimate was 0.207 for Changbai bee. The global heterozygote deficit across all populations (Fit) amounted to?0.776. About 42.3% of the total genetic variability originated from differences between breeds, with all loci contributing significantly to the differentiation. An unrooted consensus tree using the Neighbour-Joining method and pair-wise distances showed that?6 populations from Eastern China?clustered?together. The structure analysis indicated that the?6 populations were?separated first. These findings demonstrated that the 6 honeybee?populations had close genetic relationships.
机译:利用21个微卫星标记和PCR方法,研究了中国20个中华蜜蜂种群的多态性,并探讨了其遗传结构和多样性。结果表明,在842只蜜蜂中观察到507个等位基因(平均每个基因座24.14,范围在13至45之间)。乌丁蜂的杂合度最高(0.695),最低估计为长白蜂的0.207。全球所有人群的杂合子缺陷(Fit)总计为0.776。总遗传变异中约42.3%源自品种之间的差异,所有基因座均显着促进了分化。使用Neighbour-Joining方法和成对距离的无根共识树显示,来自中国东部的6个种群聚集在一起。结构分析表明,首先分离了6个种群。这些发现表明6个蜜蜂种群具有密切的遗传关系。

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