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首页> 外文期刊>African Journal of Biotechnology >Identification of polymorphism in promoter region of growth hormone receptor (GHR) gene and its association with milk related traits in Holstein cows
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Identification of polymorphism in promoter region of growth hormone receptor (GHR) gene and its association with milk related traits in Holstein cows

机译：荷斯坦奶牛生长激素受体（GHR）基因启动子区多态性的鉴定及其与奶相关性状的关联

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It is widely accepted that hormones, growth factors and other agents exert their biological effects on target tissues by binding to specific receptors on the plasma membrane. The variability in constituent sequences of growth hormone receptor (GHR) gene is very important because of its major role in mammary gland development. The aim of the present study is to detect polymorphism in promoter region of GHR gene and its association with milk related traits in Holstein cows. Blood samples were randomly collected from 93 Holstein cows, transported to the laboratory and stored at -20°C for further analysis. DNA was extracted using modified salting-out method and a fragment of 836 base pair from promoter region of GHR gene was amplified by a specific primer pairs using polymerase chain reaction (PCR). The PCR products were digested by?AluI restriction enzyme and electrophoresed on 2% agarose gel. Result of enzyme digestion for GHR gene, showed allele?AluI(-) with the fragment sizes of 14, 75 and 747, and allele?AluI(+) with the fragment sizes of 14, 75, 145 and 602 base pairs. Frequency estimation ofAluI(-) and?AluI(+) alleles were 56 and 44%, respectively, and mean of heterozygosity was 0.49. Statistical analysis showed that cows with?AluI(+) allele had significantly higher milk protein and fat percentage at first lactation compared with cows with?AluI(-/-), while there was no significant relation between different genotypes and other traits.
机译：激素，生长因子和其他试剂通过与质膜上的特定受体结合而在靶组织上发挥其生物学作用已被广泛接受。生长激素受体（GHR）基因组成序列的可变性非常重要，因为它在乳腺发育中起主要作用。本研究的目的是检测荷斯坦奶牛GHR基因启动子区域的多态性及其与牛奶相关性状的相关性。从93头荷斯坦奶牛中随机采集血液样本，运到实验室并保存在-20°C进行进一步分析。使用改良的盐析法提取DNA，并使用聚合酶链反应（PCR）通过特异性引物对扩增GHR基因启动子区域的836个碱基对的片段。用αAluⅠ限制酶消化PCR产物，并在2％琼脂糖凝胶上电泳。 GHR基因的酶消化结果显示，等位基因ΔAluI（-）的片段大小为14、75和747，等位基因ΔAluI（+）的片段大小为14、75、145和602个碱基对。 AluI（-）和ΔAluI（+）等位基因的频率估计分别为56％和44％，平均杂合度为0.49。统计分析表明，与？AluI（-/-）母牛相比，具有？AluI（+）等位基因的母牛在初次泌乳时具有较高的乳蛋白和脂肪百分比，而不同基因型与其他性状之间没有显着关系。

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