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首页> 外文期刊>African Journal of Biotechnology >Efficient plant regeneration from leaf explants of Solanum americanum
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Efficient plant regeneration from leaf explants of Solanum americanum

机译：美洲茄叶外植体的高效植物再生

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摘要

A very efficient system for direct plant regeneration from?in vitro–derived leaf explants of?Solanum americanum?was developed.?S. americanum?is a tropical plant with important medical properties. The?in vitro?procedure that was established consists of (i) induction of shoots from leaf tissue, (ii) elongation of shoots, and (iii) rooting of plantlets. The induction of shoots was achieved on Murashige and Skoog solid medium supplemented with different combinations of zeatin riboside and 1–naphthalene acetic acid or 6–benzylaminopurine and 1–naphthalene acetic acid. The best combination for plant regeneration was MS with 5.7?mM zeatin riboside and 0.11?mM 1–naphthalene acetic acid. In the second step, the shoot clumps were transferred to MS basal medium without plant growth regulators, resulting in the differentiation of most of the shoot initials into well developed shoots. In the third stage, plantlets were efficiently rooted on?half-strength MS basal medium supplemented with 58.5 mM sucrose. The rooted plants were established in soil with a 100% success rate. This system can be useful to perform further experiments to obtain transgenic plants of this species as well as for other biotechnological approaches.
机译：开发了一种非常有效的系统，用于从“美国茄属”的离体叶片外植体中直接再生植物。 americanum？是具有重要医学特性的热带植物。建立的“体外”程序包括（i）从叶片组织中诱导出芽，（ii）芽的伸长，以及（iii）小植株的生根。在Murashige和Skoog固体培养基上添加了玉米蛋白核糖核苷和1-萘乙酸或6-苄氨基嘌呤和1-萘乙酸的不同组合，可以诱导出芽。最佳的植物再生组合是MS与5.7？mM玉米素核糖苷和0.11？mM 1-萘乙酸。在第二步中，将芽苗块转移到没有植物生长调节剂的MS基础培养基上，从而使大多数芽苗初始分化为发育良好的芽。在第三阶段，将小植株有效地植根于补充了58.5 mM蔗糖的半强度MS基础培养基上。生根的植物在土壤中建立，成功率为100％。该系统可用于进行进一步的实验以获得该物种的转基因植物以及用于其他生物技术方法。

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