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Improving production of laccase from novel basidiomycete with response surface methodology

机译：响应面法提高新型担子菌漆酶的产量

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A?Mycena purpureofusca?strain screened from six?basidiomycota?fungi is well characterized in submerged fermentation for its high production of laccase and very low mycelium generation. Optimization of submerged fermentation medium for laccase production by?M. purpureofusca?was carried out with two statistical methods including Plackett-Burman (P-B) and Box-Behnken (B-B) designs. Three variables (sucrose, MgSO4?and CuSO4) were found to affect laccase production significantly by P-B screening. B-B design with three-factor at three levers was performed to explain the combined effects of the three medium constituents. The optimum medium consisted of sucrose (4.26 g/L), yeast powder (15 g/L), MgSO4?(4.83 g/L), KH2PO4(2.7 g/L), CuSO4?(5.625 mg/L) and vitamin B1 (0.1 g/L). The laccase production was increased by 1.87 folds (277.5 U/L) using this optimized medium. Furthermore, the experimental value was closed to the prediction under the optimal condition, indicating that the chosen methods were successful to determine the optimal medium components, which is the least time consuming and most effective for laccase production. The data obtained in this study may provide new insights to large-scale laccase production by?Mycena?sp.
机译：从六种担子菌真菌中筛选出的一种Mycena purpureofusca？菌株，以其高产漆酶和极低菌丝体产生而在深层发酵中具有很好的特征。用M优化产漆酶的水下发酵培养基。 purpureofusca？用两种统计方法进行，包括Plackett-Burman（P-B）和Box-Behnken（B-B）设计。通过P-B筛选发现三个变量（蔗糖，MgSO4和CuSO4）显着影响漆酶的产生。 B-B设计采用三个因素的三个因素来解释这三种培养基成分的综合作用。最佳培养基包括蔗糖（4.26 g / L），酵母粉（15 g / L），MgSO4？（4.83 g / L），KH2PO4（2.7 g / L），CuSO4？（5.625 mg / L）和维生素B1 （0.1 g / L）。使用这种优化培养基，漆酶产量提高了1.87倍（277.5 U / L）。此外，实验值接近于最佳条件下的预测值，表明所选择的方法成功确定了最佳的培养基成分，这是最耗时且对漆酶生产最有效的方法。这项研究获得的数据可能为Mycena？sp的大规模漆酶生产提供新的见解。

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