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首页> 外文期刊>African Journal of Biotechnology >Up-regulation of Robo1 in dorsal root ganglia after sciatic nerve transection in rats
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Up-regulation of Robo1 in dorsal root ganglia after sciatic nerve transection in rats

机译：大鼠坐骨神经横断后背根神经节中Robo1的上调

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Roundabout (Robo) receptors have many important roles including mediating the migration of the growth cone and neurons, promoting axonal growth and development of nerve fascicles, and guiding the growth direction of central nervous system by binding Slit. To better understand the role of?Robo?in peripheral nervous system, this study investigated the expression profile of Robo1 in the dorsal root ganglia (DRG) of adult rats following sciatic nerve transection (SNT). Adult Sprague-Dawley rats that were untreated (n = 8), or received SNT (n?= 40), were analyzed. DRG from each treatment group at days three, seven, 14, 21, and 28 post-SNT were collected and assayed by real-time PCR and immunohistochemistry. Expression of Robo1 and 2 was performed, with staining also evaluated in relation to neuron diameters. We found that both mRNA and protein levels of Robo1 were detected in normal DRG, and these levels increased following SNT. Increases were initially detected at day three post-SNT, then peaked between day seven and 14, then gradually returned to basal levels by day 21 post-SNT. Neither normal, nor SNT DRG exhibited co-localization of Robo1 and 2, and this observation was independent of neuron diameter. These results suggest that Robo1 in DRG is upregulated following sciatic nerve transection in rats.
机译：回旋处（Robo）受体具有许多重要作用，包括介导生长锥和神经元的迁移，促进轴突的生长和神经束的发育以及通过结合Slit引导中枢神经系统的生长方向。为了更好地了解Robo1在周围神经系统中的作用，本研究调查了Robo1在坐骨神经横切（SNT）后成年大鼠背根神经节（DRG）中的表达情况。分析未经治疗（n ＝ 8）或接受SNT（n≥40）的成年Sprague-Dawley大鼠。收集每个治疗组在SNT后第3天，第7天，第14天，第21天和第28天的DRG，并通过实时PCR和免疫组织化学分析。进行了Robo1和2的表达，并且还评估了与神经元直径有关的染色。我们发现在正常DRG中检测到Robo1的mRNA和蛋白质水平，并且这些水平在SNT之后增加。最初在SNT后第3天检测到增加，然后在第7天和第14天达到峰值，然后在SNT后第21天逐渐恢复到基础水平。正常和SNT DRG均未显示Robo1和2的共定位，并且该观察结果与神经元直径无关。这些结果表明，大鼠坐骨神经横断后，DRG中的Robo1上调。

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