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首页> 外文期刊>African Journal of Biotechnology >Influence of Agrobacterium rhizogenes on induction of hairy roots for enhanced production of artemisinin in Artemisia annua L. plants
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Influence of Agrobacterium rhizogenes on induction of hairy roots for enhanced production of artemisinin in Artemisia annua L. plants

机译：发根农杆菌对发根的诱导对青蒿植物青蒿素产量提高的影响

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Artemisinin production from plant tissue cultures and induction of hairy roots?in vitro?have been considered to be a promising alternative, which offer a high degree of genetic stability, grow rapidly and produce the higher spectrum of secondary metabolites than wild type plants. Hairy root cultures developed from infection of different explants of?in vitro?germinated?Artemisia?annua?L.?plant with?Agrobacterium rhizogenes?LBA 9402 strain were selected on the basis of high artemisinin content and growth. Integration of the TL-DNA (rol?gene) region of the pRi plasmid was confirmed by polymerase chain reaction (PCR) analysis of the gene located in this region.?The effect of different environmental factors like temperature, pH, cultivation media and carbon source on growth and artemisinin production were studied in shake flask cultures. Detailed batch growth and production kinetics with sugar consumption profile were also established.?Maximum volumetric productivity of 390 μg L-1?day-1?was obtained in hairy root cultures.
机译：从植物组织培养物中生产青蒿素和在体外诱导毛状根已被认为是一种有前途的替代方法，与野生型植物相比，它们提供了高度的遗传稳定性，快速生长并产生了更高谱的次级代谢产物。在青蒿素含量高和生长高的基础上，选择了发根农杆菌LBA 9402菌株在不同萌芽条件下培养出的发芽根系。通过对位于该区域的基因的聚合酶链反应（PCR）分析，证实了pRi质粒的TL-DNA（rol？gene）区域的整合。温度，pH，培养基和碳等不同环境因素的影响在摇瓶培养中研究了生长和青蒿素的来源。还建立了详细的批次生长和具有糖消耗曲线的生产动力学。在毛状根培养物中获得了最大的390μgL-1？day-1？的容积生产率。

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