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首页> 外文期刊>African Journal of Biotechnology >Molecular survey of the Texas Phoenix decline phytoplasma population in Florida, USA
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Molecular survey of the Texas Phoenix decline phytoplasma population in Florida, USA

机译：美国佛罗里达州德州凤凰城下降植物原虫种群的分子调查

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A nested polymerase chain reaction (PCR) assay was used to amplify 16S-23S intergenic spacer (IGS) region from DNA samples individually extracted from 25?Sabal palmetto?(cabbage palms) showing symptoms of Texas Phoenix decline (TPD) in West Central Florida. The IGS region was also amplified from DNA from other palm species showing symptoms of TPD and lethal yellowing (LY). A subset of the aforementioned phytoplasma DNA samples (Sabal?and other palm species) together with additional samples from various hosts collected from different geographical localities were further studied to compare the collected phytoplasma strains using sequence analysis of the glycoprotease (gcp) genes. Restriction fragment length polymorphisms (RFLP) analysis of the PCR-amplified 16S-23S IGS region and thegcp?gene using a three restriction enzymes showed that the population of the phytoplasmas infecting?S. palmetto?in West Central Florida is probably homogenous. The?S. palmetto?phytoplasma also appeared similar to all the 16SrIV-D phytoplasmas infecting other palm species and different from all phytoplasmas belonging to the 16SrIV-A subgroup. We recommend more work using genes or genomic regions other than the 16S-23S IGS region and the?gcp?gene to be done.
机译：巢式聚合酶链反应（PCR）方法用于从分别从25？Sabal palmetto？（白菜棕榈）中单独提取的DNA样品中扩增16S-23S基因间隔区（IGS）区域，该样品显示出佛罗里达州中西部的德克萨斯凤凰城下降（TPD）症状。还从其他棕榈物种的DNA中扩增了IGS区，显示出TPD和致命性黄变（LY）症状。使用糖蛋白（gcp）基因的序列分析，进一步研究了上述植物质体DNA样本（萨巴尔和其他棕榈树种）的子集，以及从不同地理位置收集的来自各种宿主的其他样品，以比较收集到的植物质体菌株。用三种限制性内切酶对PCR扩增的16S-23S IGS区域和gcpβ基因进行限制性片段长度多态性（RFLP）分析，结果表明感染αS的植原体群体。佛罗里达州中西部的palmetto？可能是同质的。 S？棕榈叶植物质体也似乎与感染其他棕榈树种的所有16SrIV-D植物质体相似，并且不同于属于16SrIV-A亚组的所有植物质体。我们建议使用16S-23S IGS区域和“ gcp”基因以外的基因或基因组区域进行更多工作。

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