Development of polyclonal antibodies for the detection of recombinant human erythropoietin

Collares Thaís Farias; Monte Leonardo Garcia; Campos Vinicius Farias; Seixas Fabiana K?mmiling; Collares Tiago Veiras; Dellagostin Odir; Hartleben Cláudia Pinho

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Development of polyclonal antibodies for the detection of recombinant human erythropoietin

机译：用于检测重组人促红细胞生成素的多克隆抗体的开发

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Recombinant human erythropoietin (rHuEPO) is detected by using direct pharmacological assays and indirect haematological assays. However, both methods have several limitations including technical challenges and cost-related issues. The aim of this study was to develop polyclonal antibodies against rHuEPO (anti-rHuEPO pAb) that can be used in immunoassays. In this study, we purified anti-rHuEPO pAb that could be used in immunoblotting assays to efficiently detect rHuEPO. Furthermore, these anti-rHuEPO pAb which could also detect rHuEPO that was expressed in a eukaryotic expression system (CHO cells). Thus, the anti-rHuEPO pAb developed in this study may be useful for rHuEPO detection.

机译：重组人促红细胞生成素（rHuEPO）通过使用直接药理测定和间接血液学测定来检测。但是，这两种方法都有一些局限性，包括技术挑战和与成本相关的问题。这项研究的目的是开发针对rHuEPO的多克隆抗体（抗rHuEPO pAb），该抗体可用于免疫分析。在这项研究中，我们纯化了抗rHuEPO pAb，可将其用于免疫印迹分析以有效检测rHuEPO。此外，这些抗rHuEPO pAb还可检测在真核表达系统（CHO细胞）中表达的rHuEPO。因此，本研究开发的抗rHuEPO pAb可能对rHuEPO检测有用。

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来源
《African Journal of Biotechnology》 |2013年第37期|共4页
作者
Collares Thaís Farias; Monte Leonardo Garcia; Campos Vinicius Farias; Seixas Fabiana K?mmiling; Collares Tiago Veiras; Dellagostin Odir; Hartleben Cláudia Pinho;
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中图分类生物工程学（生物技术）;
关键词
AntibodiesrHuEPOimmunoassayspAb;

机译：抗体HuEPO免疫分析抗体;

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专利

1. Production of novel anti-recombinant human erythropoietin monoclonal antibodies and development of a sensitive enzyme-linked immunosorbent assay for detection of bioactive human erythropoietin. [J] . Yanagihara S, Kori Y, Ishikawa R, Journal of immunoassay and immunochemistry . 2008,第2期

机译：新型抗重组人促红细胞生成素单克隆抗体的生产以及用于检测生物活性人促红细胞生成素的灵敏酶联免疫吸附测定法的开发。
2. Development and validation of a cell-based bioassay for the detection of neutralizing antibodies against recombinant human erythropoietin in clinical studies. [J] . Wei X, Swanson SJ, Gupta S Journal of Immunological Methods . 2004,第1a2期

机译：在临床研究中用于检测针对重组人促红细胞生成素的中和抗体的基于细胞的生物测定法的开发和验证。
3. Detection of anti-erythropoietin antibodies in haemodialysis patients treated with recombinant human-erythropoietin. [J] . Castelli G, Famularo A, Semino C, Pharmacological research: The official journal of The Italian Pharmacological Society . 2000,第3期

机译：在重组人促红细胞生成素治疗的血液透析患者中检测抗促红细胞生成素抗体。
4. IMMUNO-PROTEOMICS USING POLYCLONAL ANTIBODIES AND STABLE ISOTOPE LABELED AFFINITY-PURIFIED RECOMBINANT PROTEIN FRAGMENTS [C] . Fredrik Edfors, Tove Bostrom, Bjorn Forsstrom, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

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5. Recombinant polyclonal antibody libraries for breast cancer therapy. [D] . Santora, Kenneth Edward. 1998

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6. From Polyclonal Sera to Recombinant Antibodies: A Review of Immunological Detection of Gluten in Foodstuff [O] . Eduardo Garcia-Calvo, Aina García-García, Raquel Madrid, 2021

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7. Human kallikrein 13: production and purification of recombinant protein and monoclonal and polyclonal antibodies, and development of a sensitive and specific immunofluorometric assay [O] . Carl Kapadia, Albert Chang, Georgia Sotiropoulou, 2003

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Development of polyclonal antibodies for the detection of recombinant human erythropoietin

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