Production of -amylase by solid state fermentation by Rhizopus oryzae

Osania Emerenciano Ferreira; Nayara Abrao Montijo; Eduardo da Silva Martins; Marcia Justino Rossini Mutton

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Production of -amylase by solid state fermentation by Rhizopus oryzae

机译：米根霉固态发酵生产α-淀粉酶

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A thermotolerant strain of Rhizopus oryzae was grown in three agro-industrial by-products: brewers’ rice, corn grits and wheat bran. Different substrates, cultivation time, moisture content, additional nitrogen sources, pH and temperature of incubation were evaluated aiming to optimize growing conditions. The highest enzymatic activity was observed after 24 h of cultivation using wheat bran as substrate with the following salt solutions: NH4NO3, MgSO4.7H2O and (NH4)2SO4 0.1% at temperature of 35°C. It was observed that changes in the pH range 4.0-6.0 did not significantly affect α-amylase activity. The optimum operation conditions were 75°C and pH 4.5. The enzymes remained stable at 75°C in the absence of substrate for 25 min.

机译：在三种农用工业副产品中种植了耐热的米根霉菌株：啤酒的大米，玉米corn和麦麸。评估了不同的底物，培养时间，水分含量，额外的氮源，pH和孵化温度，旨在优化生长条件。使用小麦麸作为底物，在以下盐溶液下培养24小时后，观察到最高的酶活性：NH4NO3，MgSO4.7H2O和0.1％（NH4）2SO4，温度为35°C。观察到pH在4.0-6.0范围内的变化不会显着影响α-淀粉酶活性。最佳操作条件是75°C和pH 4.5。在没有底物的情况下，酶在75°C保持稳定25分钟。

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来源
《African Journal of Biotechnology》 |2015年第7期|共7页
作者
Osania Emerenciano Ferreira; Nayara Abrao Montijo; Eduardo da Silva Martins; Marcia Justino Rossini Mutton;
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中图分类生物工程学（生物技术）;
关键词
Starch hydrolysisfermentation parametersamylolytic enzyme;

机译：淀粉水解发酵参数淀粉分解酶;

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Production of -amylase by solid state fermentation by Rhizopus oryzae

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