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首页> 外文期刊>African Journal of Biotechnology >Genetic diversity analysis of mustard (Brassica spp.) germplasm using molecular marker for selection of short duration genotypes
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Genetic diversity analysis of mustard (Brassica spp.) germplasm using molecular marker for selection of short duration genotypes

机译：芥菜（Brassica spp。）种质的遗传多样性分析，使用分子标记选择短时基因型

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Molecular characterization of 16 mustard (Brassica spp.) genotypes by using 12 RAPD markers revealed that three primers GLA-11, OPB-04 and OPD-02 showed good technical resolution and sufficient variations among different genotypes. A total of 40 RAPD bands were scored of which 38 (94.87%) polymorphic amplification products were obtained. Besides, the primer OPD-02 amplified maximum number of polymorphic bands (100.00%) while the primer GLA-11 and OPB-04 generated the least (92.31%) polymorphic bands, which were minimal in number. The present study produced 13.33 scorable bands per primer and 12.67 polymorphic bands per primer. Frequencies of maximum number of polymorphic loci were found to be high with the exception of GLA-11(0.750), OPB-04 (0.875) and OPD-02 (0.750). The estimate of Nei's genetic diversity for the entire genotypes of mustard was 0.3596 and Shannon's information index was 0.535. There was a high level of genetic variation among the mustard genotypes studied from the proportion of polymorphic loci point of view. The values of pair-wise comparison of Nei’s genetic distance between genotypes were computed from combined data for the three primers; ranged from 0.1054 to 0.9862. BINA Sarisha-3 and BINA Sarisha-4 showed the lowest genetic distance of 0.1054 where Tori-7 and NAP-0758-2 showed highest genetic distance of 0.9862. The 16 mustard genotypes were differentiated into three main clusters: BARI Sarisha-14, BARI Sarisha-9, BARI Sarisha-15, BINA Sarisha-4, BINA Sarisha-3, BARI Sarisha-8, Sampad and Tori-7 in cluster A, NAP-0763, NAP-0721-1, BARI Sarisha-4, BARI Sarisha-6, NAP-0762-2 and NAP-0848-2 in cluster B and NAP-0838 and NAP-0758-2 were grouped into cluster C by making dendrogram based on Nei’s genetic distance using unweighted pair group method of arithmetic means (UPGMA).
机译：通过使用12种RAPD标记对16个芥菜（Brassica spp。）基因型进行分子表征，发现三种引物GLA-11，OPB-04和OPD-02显示出良好的技术分辨率，并且在不同基因型之间具有足够的变异性。总共对40条RAPD谱带进行了评分，其中获得了38种（94.87％）多态性扩增产物。此外，引物OPD-02扩增出最多的多态性条带（100.00％），而引物GLA-11和OPB-04产生了最少（92.31％）的多态性条带，数量最少。本研究产生每个引物13.33个可评分带和每个引物12.67个多态性带。发现最大多态位点的频率高，除了GLA-11（0.750），OPB-04（0.875）和OPD-02（0.750）。 Nei整个芥菜基因型的遗传多样性估计为0.3596，Shannon的信息指数为0.535。从多态性基因座的比例来看，芥菜基因型之间存在较高的遗传变异。根据三种引物的组合数据，计算基因型之间Nei遗传距离的成对比较值;范围从0.1054到0.9862。 BINA Sarisha-3和BINA Sarisha-4的最低遗传距离为0.1054，其中Tori-7和NAP-0758-2的最高遗传距离为0.9862。芥菜的16种基因型被分为三个主要群：群A中的BARI Sarisha-14，BARI Sarisha-9，BARI Sarisha-15，BINA Sarisha-4，BINA Sarisha-3，BARI Sarisha-8，Sampad和Tori-7。群集B中的NAP-0763，NAP-0721-1，BARI Sarisha-4，BARI Sarisha-6，NAP-0762-2和NAP-0848-2以及群集NAP-0838和NAP-0758-2通过利用Nei的遗传距离，使用算术平均数的非加权成对分组法（UPGMA）来制作树状图。

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