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首页> 外文期刊>Indian Journal of Agricultural Biochemistry >Assessment of Genetic Diversity in Mustard (Brassica juncea L.) Genotypes using Biochemical and Molecular Markers
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Assessment of Genetic Diversity in Mustard (Brassica juncea L.) Genotypes using Biochemical and Molecular Markers

机译：利用生化和分子标记评估芥菜（Brassica juncea L.）基因型的遗传多样性

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摘要

Indian mustard (Brassica juncea L.) is an important source of edible oil in India. Quality parameters such as moisture, oil, protein, erucic acid and sinigrin were analysed in sixty genotypes of mustard [Brassica juncea L.) and from which 15 genotypeswith desired proximate parameters were selected and analysed further for anti nutritional factors, genetic diversity and isozymes. Erucic acid and Glucosinolate (Sinigrin) was analysed by gas chromatography- mass spectrometry (GC-MS) and ultra performance liquid chromatography (UPLC), respectively. SKM 9033 showed the highest (30.88 %) oil where as RAYAD-9602 had the lowest (20.80 %) oil. The protein content and true protein in mustard cultivars ranged from 27.90-33.69% and 10.66-20.01%, respectively.HNT-33 genotype had the highest concentration of erucic acid where as NRCM-120 showed the lowest. Bio-Q-44-279 had the highest sinigrin concentration (46.80 umole'g) while SKM-9033 had the lowest sinigrin concentration (2.49 umole/g). Isozyme pattern ofperoxidase and esterase showed significant correlation amongst the clusters. Electrophoresis of seed protein by SDS-PAGE showed a total of 28 bands with varied intensity in mustard genotypes. Both Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) markers were found to be efficient to assess genetic diversity within species of mustard with average polymorphism of 82.91% and 96.02%, respectively. NRCM-120 and SKM-9033 had low content of erucic acid and sinigrin respectively and hence were found to be useful for the development of double zero variety by breeding program. The SW-1-9017 and Bio-341-92 genotypes showed high genetic diversity compared to other genotypes and found to be useful for breeding purpose.
机译：印度芥菜（Brassica juncea L.）是印度食用油的重要来源。分析了芥菜[Brassica juncea L.]的60个基因型中的质量参数，例如水分，油，蛋白质，芥酸和芥子苷，并从中选择了15种具有所需邻近参数的基因型，并进一步分析了其抗营养因子，遗传多样性和同工酶。分别通过气相色谱-质谱（GC-MS）和超高效液相色谱（UPLC）分析芥酸和葡萄糖苷（Sinigrin）。 SKM 9033的油品含量最高（30.88％），而RAYAD-9602的油品含量最低（20.80％）。芥菜品种的蛋白质含量和真蛋白含量分别为27.90-33.69％和10.66-20.01％.HNT-33基因型的芥酸浓度最高，而NRCM-120最低。 Bio-Q-44-279的芥子苷浓度最高（46.80 umole'g），而SKM-9033的芥子苷浓度最低（2.49 umole / g）。各簇之间过氧化物酶和酯酶的同工酶模式显示出显着的相关性。通过SDS-PAGE电泳种子蛋白显示共有28条带，芥菜基因型强度不同。发现简单序列重复（SSR）和简单序列间重复（ISSR）标记均可有效评估芥菜种内的遗传多样性，平均多态性分别为82.91％和96.02％。 NRCM-120和SKM-9033的芥酸和芥子苷含量较低，因此可通过育种程序用于开发双零品种。与其他基因型相比，SW-1-9017和Bio-341-92基因型显示出较高的遗传多样性，并被发现可用于育种目的。

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