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首页> 外文期刊>African Journal of Biotechnology >Glyoxalase I expression pattern in Hevea brasiliensis seedlings under varied stress conditions
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Glyoxalase I expression pattern in Hevea brasiliensis seedlings under varied stress conditions

机译：不同胁迫条件下巴西橡胶树幼苗中乙二醛酶I的表达模式

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Drought is one of the most important stress factors which adversely affect plants’ growth and productivity. Global climate change may make this situation more serious in the years ahead.? Considering the long time span required for the generation of drought resistant genotypes in Rubber (Hevea brasiliensis) through conventional breeding, molecular interventions to engineer plants to have either drought responsive genes or genes expected to alter osmotic regulation would be very attractive. The glyoxalase pathway involving glyoxalase I and glyoxalase II enzymes is required for glutathione-based detoxification of methylglyoxal. In this study the effects of various abiotic stresses on the up-regulation of methylglyoxal levels and glyoxalase I activities in Hevea brasiliensis seedlings were investigated. Most of the stresses caused significant increase in methylglyoxal level and glyoxalase I activity, among which drought caused the highest induction of glx I followed by salinity, 2, 4-D, ABA, methylglyoxal, white light and CdCl2. The stress-induced increases in methylglyoxal and glyoxalase I activity found in the present study suggest an important role of glyoxalase I in conferring drought tolerance. The up-regulation of glyoxalase I under drought stress indicates its future utility in developing tolerance to drought stress in Hevea brasiliensis. In the present study, a partial cDNA sequence coding for glyoxalase I was amplified by PCR using specific primers. The 440 bp cDNA amplicon obtained was sequenced and subjected to online BLAST analysis. The sequence of Hevea brasiliensis glyoxalase I (GenBank Acc. No: GU598520) had six open reading frames. The ORF finder revealed the longest ORF of 336 bp. Glyoxalase I from Ricinus communis had the highest nucleotide sequence homology (90%) compared to the amplified gene. BLASTP analysis also showed high homology between the deduced protein sequence of the amplified gene and glyoxalases from other species. Our results suggest that the multi-stress inducibility of glyoxalase I in the present study may be due to the fact that it might protect the plants against MG that is formed under various stresses including drought and confers tolerance by increasing the GSH-based detoxification system and decreasing lipid peroxidation.
机译：干旱是对植物生长和生产力产生不利影响的最重要的胁迫​​因素之一。全球气候变化可能使这种情况在未来几年变得更加严重。考虑到通过常规育种在橡胶（巴西橡胶树）中产生抗旱基因型需要很长的时间跨度，因此对植物进行分子干预以使其具有干旱响应基因或预期会改变渗透调节的基因将非常具有吸引力。涉及乙二醛酶I和乙二醛酶II的乙二醛酶途径是基于谷胱甘肽的甲基乙二醛解毒所必需的。在这项研究中，研究了各种非生物胁迫对巴西橡胶树幼苗甲基乙二醛水平和乙二醛酶I活性上调的影响。大多数胁迫导致甲基乙二醛水平和乙二醛酶I活性显着增加，其中干旱导致对glx I的诱导最高，其次是盐度，2、4-D，ABA，甲基乙二醛，白光和CdCl2。在本研究中发现，应力诱导的甲基乙二醛和乙二醛酶I活性增加表明乙二醛酶I在赋予抗旱性中具有重要作用。干旱胁迫下乙二醛酶I的上调表明其在发展巴西橡胶树抗旱性中的未来用途。在本研究中，使用特异性引物通过PCR扩增了编码乙二醛酶I的部分cDNA序列。对获得的440 bp cDNA扩增子进行测序，然后进行在线BLAST分析。巴西橡胶树乙二醛酶I（GenBank编号：GU598520）的序列具有六个开放阅读框。 ORF查找器显示最长的336 bp ORF。与扩增的基因相比，来自蓖麻的乙二醛酶I具有最高的核苷酸序列同源性（90％）。 BLASTP分析还显示，推导的扩增基因的蛋白质序列与来自其他物种的乙二醛酶之间具有高度同源性。我们的结果表明，本研究中乙二醛酶I的多胁迫诱导性可能是由于它可以保护植物免受在各种胁迫（包括干旱）下形成的MG的侵害，并通过增加基于GSH的排毒系统赋予植物耐性。减少脂质过氧化。

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