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首页> 外文期刊>African Journal of Biotechnology >Hepatoxicity of aqueous extract and fractionated methanol extract of Phytolacca americana by isolated rat liver perfusion system
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Hepatoxicity of aqueous extract and fractionated methanol extract of Phytolacca americana by isolated rat liver perfusion system

机译：分离的大鼠肝脏灌流系统提取美国醉草水提物和甲醇分馏物的肝毒性。

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Phytolacca americana?is a perennial plant native to North America and other parts of the world. It is well known for several medicinal properties despite being considered to have digestive toxicity (especially hepatotoxicity). Our objective is to examine whether extract of aerial parts of?P. americana?could produce biochemical changes by Isolated Rat Liver Perfusion (IRLP) system, which is ideal for studying biochemical alterations of chemicals with minimum neural-hormonal effects. In this study, the liver was perfused with Kerbs-Henseleit buffer, containing different concentration of aqueous extract of the aerial parts of?P. americana?(10, 20, 40, 50,100 mg/kg) and CHCl3, ethanol and methanol fraction (10, 20, 40 mg/kg) were added to the buffer and perfused for 2 h. During the perfusion, many factors including amino-transferase activities were assessed as indicator of liver viability. Consequently, sections of liver tissue were examined for any histo-pathological changes. The results showed that histo-pathological changes in liver tissues were related in a dose-dependent manner to methanol extract of aerial parts?Phytolacca americana?concentrations. Doses of 50, 100 mg/kg caused significant (P < 0.05) histo-pathological changes.
机译：美洲疫霉是多年生植物，原产于北美和世界其他地区。尽管被认为具有消化毒性（尤其是肝毒性），但它具有多种医学特性。我们的目标是检查是否提取了吗啡的气生部分。 Americana可以通过离体大鼠肝灌注（IRLP）系统产生生物化学变化，这对于研究具有最小神经激素作用的化学物质的化学变化是理想的。在这项研究中，肝脏用Kerbs-Henseleit缓冲液灌注，缓冲液中含有不同浓度的？P空中部分的水提物。将美洲？（10、20、40、50,100 mg / kg）和CHCl3，乙醇和甲醇级分（10、20、40 mg / kg）添加到缓冲液中，并灌注2 h。在灌注过程中，包括氨基转移酶活性在内的许多因素被评估为肝生存能力的指标。因此，检查肝组织切片是否有任何组织病理学变化。结果表明，肝组织的组织病理学改变与地上部分甲醇提取物的浓度有关。剂量为50、100 mg / kg会引起显着（P <0.05）的组织病理学改变。

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