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Bioconversion of rape straw into a nutritionally enriched substrate by Ganoderma lucidum and yeast

机译:灵芝和酵母将油菜秸秆转化为营养丰富的底物

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This work aims to select biological treatments and conditions for the bioconversion of rape straw by the mixed-strain fermentation of?Ganoderma lucidum?and yeasts (Saccharomyces cerevisiae, Candida tropicalis and Candida utilis), into an enriched substrate with increased?crude protein?and digestibility. Orthogonal experiment showed that?the optimal experimental condition for the crude protein enrichment was:10%?(v/w)?C. utilis?inoculum was added to the rape straw medium after 7 days of?G.lucidum?growth; the crude protein content of the?substrate was?16.23%; the yield rate were increased by 75.70 and 225.90%, respectively when compared to the?G.lucidum?individual-fermentation and without fermentation substrate.?The results in this study also indicated that:?the co-culture of 2 fungi (G.?lucidum?+?C.?utilis) was better than individual (G.?lucidum) culture on the?degradation of cellulose and lignin of rape straw substrate and the secretion of ligninolytic enzyme system including laccase (Lac), manganese peroxidase (MnP) ?and lignin peroxidase (LiP).
机译:这项工作的目的是通过“灵芝”和酵母(酿酒酵母,热带假丝酵母和utild假丝酵母)的混合菌株发酵,为油菜秸秆的生物转化选择生物处理方法和条件,使其成为富含粗蛋白的富集底物。可消化性。正交实验表明,粗蛋白富集的最佳实验条件为:10%?(v / w)?C。灵芝生长7天后,将ino草菌加入油菜秸秆培养基中。底物的粗蛋白含量为16.23%。与单独发酵和不加发酵底物的G.lucidum发酵相比,产量分别提高了75.70%和225.90%。本研究的结果还表明:2种真菌的共培养(G.在油菜秸秆底物的纤维素和木质素的降解以及漆酶(Lac),锰过氧化物酶(MnP)的木质素分解酶系统的分泌上,“灵芝” +“ C.utilis”比单独培养的“灵芝”更好。 )和木质素过氧化物酶(LiP)。

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