In vitro regeneration of Pakistani peanut (Arachis hypogea L.) varieties using de-embryonated coteledonary explants

Farhat Nazir; Mahmood-ul-Hassan; Zahid Akram; Muhammad Matloob Javed; Shoukat Ali; Ghulam Muhammad Ali; Yusuf Zafar

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In vitro regeneration of Pakistani peanut (Arachis hypogea L.) varieties using de-embryonated coteledonary explants

机译：用去胚的子叶外植体体外再生巴基斯坦花生（Arachis hypogea L.）品种

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Peanut (Arachis hypogaea?L.) belongs to the family leguminosae and is one of the world’s largest oilseed crops. This study is the first report on peanut regeneration from Pakistan using four commercially released peanut varieties, that is, Golden, BARI-2000, BARD-479 and BARD-92. Longitudinally, halved cotyledons with removed embryos were employed as explants. Among various tested combinations of BAP and NAA, the best combination was 4 and 0.1 mg/L respectively. BARI-2000 proved to be the best responsive variety for?in vitro?regeneration in terms of number of shoots/explant (133.3%) and number of rooted plants/explant (124.99%). The optimized protocol, which is the first one in the country, would be used to incorporate important traits in future breeding programmes of peanut in Pakistan.

机译：花生（花生）属于豆科，是世界上最大的油料作物之一。这项研究是有关巴基斯坦使用四种商业发行的花生品种Golden，BARI-2000，BARD-479和BARD-92进行花生再生的第一份报告。纵向上，将一半的子叶和去除的胚用作外植体。在BAP和NAA的各种测试组合中，最佳组合分别为4和0.1 mg / L。就芽/植株数（133.3％）和生根的植株/植株数（124.99％）而言，BARI-2000被证明是体外再生的最佳响应品种。经过优化的方案是该国的第一个方案，将用于在巴基斯坦未来的花生育种计划中纳入重要的性状。

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《African Journal of Biotechnology》 |2011年第43期|共6页
作者
Farhat Nazir; Mahmood-ul-Hassan; Zahid Akram; Muhammad Matloob Javed; Shoukat Ali; Ghulam Muhammad Ali; Yusuf Zafar;
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中图分类生物工程学（生物技术）;
关键词
Arachis hypogeade-embryonated cotyledonsBAPNAAin vitroregenerationPakistan;

机译：花生次子代杂交的子叶BAPNAA体外再生;

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1. Factors promoting efficient in vitro regeneration from de-embryonated cotyledon explants of Arachis hypogaea L. [J] . Siddharth Tiwari, Rakesh Tuli Plant Cell, Tissue and Organ Culture . 2008,第1期

机译：促进花生无子叶子叶外植体高效再生的因素
2. Factors promoting efficient in vitro regeneration from de-embryonated cotyledon explants of Arachis hypogaea L. [J] . Tiwari S, Tuli R Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants . 2008,第1期

机译：促进花生无子叶子叶外植体有效再生的因素
3. Surface sterilant effect on the regeneration efficiency from cotyledon explants of groundnut (Arachis hypogea L.) varieties adapted to eastern and Southern Africa [J] . Susan Muthoni Maina, Quinata Emongor, Kiran K. Sharma, African Journal of Biotechnology . 2010,第20期

机译：表面消毒剂对适应东部和南部非洲的花生（Arachis hypogea L.）子叶外植体再生效率的影响
4. Evaluation of peanut (Arachis hypogaea L.) varieties in the lower Markham Valley of Papua New Guinea [C] . Humphrey Saese, Geoff Fahey, John Bafui Workshop of the Australian Centre for International Agricultural Research . 2006

机译：巴布亚新几内亚下马克姆谷的花生（Arachis Hypogaea L.）品种评价
5. Flavor formation and sensory perception of selected peanut genotypes (Arachis hypogea L.) as affected by storage water activity, roasting, and planting date. [D] . Baker, George Leroy, IV. 2002

机译：受贮水活度，焙烧和播种日期的影响，所选花生基因型（花生）的风味形成和感官知觉。
6. Marker-assisted introgression of a QTL region to improve rust resistance in three elite and popular varieties of peanut (Arachis hypogaea L.) [O] . Rajeev K. Varshney, Manish K. Pandey, Pasupuleti Janila, -1

机译：标记辅助渗入QTL区域以提高三种优良和受欢迎的花生品种（Arachis hypogaea L.）的耐锈性
7. In vitro regeneration of Pakistani peanut (Arachis hypogea L.) varieties using de-embryonated coteledonary explants [O] . Nazir Farhat, Akram Zahid, Matloob Javed Muhammad, 2011

机译：使用De-Engrouned Coteledonary外植体的Pakistani花生（Arachis Hypofea L.）品种的体外再生

In vitro regeneration of Pakistani peanut (Arachis hypogea L.) varieties using de-embryonated coteledonary explants

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