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首页> 外文期刊>African Journal of Biotechnology >Identification and sequence analysis of pyrokinin/PBAN peptide of psocids, Liposcelis entomophila
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Identification and sequence analysis of pyrokinin/PBAN peptide of psocids, Liposcelis entomophila

机译：昆虫嗜脂单胞菌嗜热菌素/ PBAN肽的鉴定和序列分析

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摘要

Using an antiserum against?Helicoverpa zea?pheromone biosynthesis activating neuropeptide (PBAN), pyrokinin/PBAN-like immunoreactivity in the head and body of female of?Liposcelis entomophila?was detected by competitive ELISA. Pyrokinin/PBAN peptides were extracted and purified by reverse-phase HPLC, and the sequence were elucidated by a matrix-assisted laser desorption ionization time of flight mass spectrometer (MALDI-MS-TOF). The results showed that pyrokinin/PBAN neuropeptide was expressed in the heads of?L. entomophilafemales. Meanwhile, it was not found in the abdomen of?L. entomophila?females and the head of?Liposcelis?bostrychophila.?These results indicated that the peptide perhaps had the functions to regulate sex pheromone releasing. The sequence pyrokinin/PBAN family peptide in?L. entomophila?was SAGMFTPRL -NH2. The result of BLASTp in NCBI web showed that its sequence had largely homologous sequences with other PBAN/pyrokinin peptides sequence in other insects that were published and share a highly conserved sequence FXPRLamide in the C terminal. This result indicated that the identified peptide in?L. entomophila?belong to the pyrokinin/PBAN family. The purification and identification of this peptide have an important instructional meaning for cloning the gene of pyrokinin/PBAN and elucidating the release mechanism of the sex pheromone of?L. entomophila.
机译：通过竞争性ELISA，使用针对“ Helicoverpa zea”信息素生物合成激活神经肽（PBAN）的抗血清，检测了“嗜热脂单胞菌”（Liposcelis entomophila）雌性头部和体内的焦激酶/ PBAN样免疫反应性。通过反相HPLC提取并纯化焦磷酸激肽/ PBAN肽，并通过基质辅助激光解吸电离飞行时间质谱仪（MALDI-MS-TOF）阐明序列。结果表明，焦磷酸激酶/ PBAN神经肽在？L的头部表达。嗜虫菌丝。同时，在腹部未发现。结果表明，该肽可能具有调节性信息素释放的功能。序列中的pyrokininin / PBAN家族肽序列。食虫是SAGMFTPRL -NH2。 BLASTp在NCBI网站上的结果表明，其序列与已发表的其他昆虫中的其他PBAN /焦磷酸肽肽序列具有很大的同源性，并在C末端共享高度保守的序列FXPRLamide。该结果表明鉴定出的肽在ΔL中。食虫菌属于pyrokinin / PBAN家族。该肽的纯化和鉴定对于克隆pyrokinin / PBAN基因，阐明ΔL性信息素的释放机制具有重要的指导意义。食虫。

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