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首页> 外文期刊>African Journal of Biotechnology >Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of crude extracted insecticidal crystal proteins of Bacillus thuringiensis and Brevibacillus laterosporus
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Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of crude extracted insecticidal crystal proteins of Bacillus thuringiensis and Brevibacillus laterosporus

机译：苏云金芽胞杆菌和枯草芽孢杆菌粗提杀虫晶体蛋白的十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）

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摘要

Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was conducted to determine the number and size of the insecticidal crystal proteins (ICPs) synthesized by four isolates of entomopathogenic spore-forming bacteria. Only three of the four bacteria produced ICPs with SDS-PAGE profiles, of which two were strains of?Bacillus thuringiensis?and one was a strain of?Brevibacillus laterosporus. Large sized proteins bands were present in the range of 73 kDa to 135 kDa for the?B. thuringiensis?strains. All the three isolates had smaller proteins in the range of 13 kDa to 44 kDa. One strain of?B. thuringiensis, NDR11,?produced an unusual large protein of 200 kDa. Small proteins in the range of 24 kDa to 40 kDa were produced by?B. laterosporus.?The Cry ICPs of isolate NDR3 did not produce any electrophoretic bands, indicating that its ICP did not dissolve under the conditions used.
机译：进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE），以确定由四种病原性孢子形成细菌分离物合成的杀虫晶体蛋白（ICPs）的数量和大小。在四种细菌中，只有三种产生具有SDS-PAGE谱的ICP，其中两种是苏云金芽孢杆菌菌株，一种是晚孢菌株。对于βB，存在较大的蛋白质条带，范围为73kDa至135kDa。苏云金菌株。这三个分离株均具有较小的蛋白质，范围为13 kDa至44 kDa。一株？B。苏云金芽孢杆菌NDR11产生了200 kDa的异常大蛋白。 βB产生24kDa至40kDa范围内的小蛋白。分离物NDR3的Cry ICP不会产生任何电泳带，表明其ICP在所用条件下不会溶解。

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