• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Effects of Geven root extract on proliferation of HeLa cells and bcl-2 gene expressions
【24h】

Effects of Geven root extract on proliferation of HeLa cells and bcl-2 gene expressions

机译：Geven根提取物对HeLa细胞增殖和bcl-2基因表达的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Geven (Astragalus?L.) root extract is used for asthma, diarrhea, and cancer therapy in Chinese Medicine. Liquid?Astragalus?root extracts are traditionally used in Anatolia for leukemia and wound healing. 439 species of this plant, which has 3000 species in the world, grow in Turkey and 204 of these? are endemic. In this study, molecular mechanism of apoptotic and cytotoxic effects of root ethyl acetate extract ofAstragalus chrysochlorus?on HeLa cells was investigated.?For this purpose, 1, 0.5, 0.15 and 0.1 mg/ml concentrations of?Astragalus?root extract were applied to HeLa cell cultures for 24, 48 and 72 h. Cell kinetic parameters as proliferation rate (MTT assay), Mitotic Index and apoptotic index were used for experimental cytotoxicity assay of root extract. Furthermore, effects of root extract on gene expression rates which take place in apoptosis mechanism as?Bcl-2, Bax, Bak, Bcl-x, Bik, Mcl-1, Bfl-1were determined by reverse transcription-polymerase chain reaction (RT-PCR) technique. As a result of investigated parameters, root extract of?Astragalus chrysochlorus?is found to have cytotoxic effect on HeLa cell cultures. This cytotoxic effect which appeared in our experiments achieved statistically significant decrease on proliferation rate of HeLa cells depending on application time and concentration. Also mitotic index and apoptotic index presented statistically significant increase on particular concentrations and hours (p < 0.05). Likewise, it is determined on molecular basis that root extract causes significant changes on especially antiapoptotic genes.
机译：Geven（Astragalus？L。）根提取物用于中药治疗哮喘，腹泻和癌症。液体“黄芪”根提取物传统上在安纳托利亚用于白血病和伤口愈合。这种植物有439种，在世界上有3000种，其中有204种？是地方性的。本研究研究了黄芪根乙酸乙酯提取物对HeLa细胞凋亡和细胞毒性作用的分子机理。为此，分别以1、0.5、0.15和0.1 mg / ml浓度的黄芪根提取物用于HeLa细胞。 HeLa细胞培养24、48和72小时。细胞动力学参数如增殖率（MTT测定），有丝分裂指数和凋亡指数用于根提取物的实验性细胞毒性测定。此外，通过逆转录-聚合酶链反应（RT-RT）确定了根提取物对在凋亡机制中发生的基因表达速率的影响，如βBcl-2，Bax，Bak，Bcl-x，Bik，Mcl-1，Bfl-1。 PCR）技术。作为研究参数的结果，发现黄芪的根提取物对HeLa细胞培养具有细胞毒性作用。根据应用时间和浓度，在我们的实验中出现的这种细胞毒性作用在统计学上显着降低了HeLa细胞的增殖速率。在特定浓度和小时下，有丝分裂指数和凋亡指数也有统计学上的显着增加（p <0.05）。同样，基于分子确定根提取物引起特别是抗凋亡基因的显着变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号