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首页> 外文期刊>African Journal of Biotechnology >Experimental analysis on the effect of addition of Rhizoma gastrodiae on mycelia and exopolysaccharide productions by submerged culture of Grifola frondosa
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Experimental analysis on the effect of addition of Rhizoma gastrodiae on mycelia and exopolysaccharide productions by submerged culture of Grifola frondosa

机译：灰树花深层培养添加天麻根对菌丝体和胞外多糖产生影响的实验分析

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摘要

The effect of addition of?Rhizoma?gastrodiae?on mycelia and exopolysaccharide production by submerged culture of?Grifola?frondosa?was investigated. About 7% (v/v) of?R. gastrodiae?extract was added to the basal medium used for its biomass and exopolysaccharide productions. The biomass and exopolysaccharide productions reached a maximum of 2.0630±0.0520 g/L and 89.3846±3.2422 mg/L, respectively after day 10 and 8 of cultivation.?G. frondosa?was sensitive to?R. gastrodiae, and there was a reduction in the cycle of exopolysaccharide synthesis. The pH variation in culture provided an indirect inference to this stimulating effect of?R. gastrodiae?extractaddition. Moreover, a curve fitting and parameter analysis was carried out to assay the increasing effect on biomass and exopolysaccharide productions. Meanwhile, the kinetics parameters predicted through the fitting equations in culture, X0?= 0.1523 g/L, Xm?= 2.0630 g/L, μm?= 0.4994 d-1, α = 0.0858 and β = -0.0069, further clarified the stimulatory effects of?R. gastrodiae?extract?addition in?G. frondosa?culture.
机译：研究了添加“天麻根瘤菌”对深层培养的“灰树花”植物的菌丝体和胞外多糖的影响。约7％（v / v）的R.将天麻提取物添加到用于其生物质和胞外多糖生产的基础培养基中。培养第10天和第8天后，生物量和胞外多糖的最高产量分别达到2.0630±0.0520 g / L和89.3846±3.2422 mg / L。 frondosa？对？R敏感天麻，并减少了胞外多糖合成的周期。培养中pH的变化间接暗示了ΔR的这种刺激作用。天麻提取物。此外，进行了曲线拟合和参数分析，以测定对生物量和胞外多糖产量的增加作用。同时，通过培养中的拟合方程预测的动力学参数X0≤0.1523g/ L，Xm≤2.0630g/ L，μm≤0.4994d-1，α＝ 0.0858，β＝ -0.0069，进一步阐明了刺激性。 R的影响天麻提取物的添加frondosa？文化。

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