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首页> 外文期刊>African Journal of Biotechnology >Effect of AgNO3 on androgenesis of Brassica oleracea L. anthers cultivated in vitro
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Effect of AgNO3 on androgenesis of Brassica oleracea L. anthers cultivated in vitro

机译：AgNO3对甘蓝型油菜花药雄性发育的影响

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The present article is a synthesis study accomplished at Vegetable Research and Development Station Bacau regarding the implication of silver nitrate?(AgNO3)?in modulating the morphogenetic reaction of white cabbage anthers cultivated?in vitro. According to literature, silver nitrate is a strong inhibitor of ethylene action. Embryo formation, shoot generation and rooting of plantlets are key points on which relies the entire concept of?in vitro?tissue culture. Silver ions, in the form of nitrate play an important role in promoting the somatic embryogenesis and organogenesis, which led its wide spread use in different plant tissue culture?in vitro. Therefore, our researches focused on testing different concentrations of AgNO3?(5, 10, 15, 25, 30, 50 and 60 μM) added to a pre-tested medium formula–?Murashige and Skoog (MS), 1962 basal medium supplemented with benzylaminopurine?(BAP)- 8.9 μM and naphthylacetic acid(NAA)?2.7 μM. The biological material is represented by unopened flower buds containing anthers with microspores at late uninucleate to binucleate stage. In the culture condition investigated in this study, the data obtained promotes the utilization of AgNO3?in a concentration of 50 μM for the successfully induction and sustaining of regenerative processes of white cabbage anthers cultivated?in vitro.
机译：本文是在蔬菜研究与开发站Bacau完成的一项合成研究，涉及硝酸银（AgNO3）？调控体外培养的白菜花药的形态发生反应。根据文献，硝酸银是乙烯作用的强抑制剂。胚的形成，枝条的产生和小植株的生根是关键点，而这取决于整个“体外”组织培养的概念。硝酸盐形式的银离子在促进体细胞胚发生和器官发生中起重要作用，从而使其在体外不同植物组织培养中得到广泛应用。因此，我们的研究重点是测试添加到预先测试的培养基配方中的不同浓度的AgNO3？（5、10、15、25、30、50和60μM）-Murashige和Skoog（MS），1962年补充了基础培养基苄氨基嘌呤（BAP）-8.9μM和萘乙酸（NAA）-2.7μM。该生物材料的特征是未打开的花蕾，其中包含花药，花药在单核到双核的晚期具有小孢子。在这项研究中研究的培养条件下，获得的数据促进了浓度为50μM的AgNO3的利用，成功地诱导和维持了体外培养的白菜花药的再生过程。

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