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首页> 外文期刊>African Journal of Biotechnology >Characterization of Aspergillus species associated with commercially stored triphala powder
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Characterization of Aspergillus species associated with commercially stored triphala powder

机译：与商业储存的三叶草粉相关的曲霉菌种的表征

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About 82 triphala powder samples were analyzed for the association of different fungi. Results reveal the predominance of?Aspergillus?as the major genera with six predominant species namely,?A. niger, A. flavus, A. fumigatus, A. terreus, A. nidulansand?A. amstelodami. Therefore, these six isolated?Aspergillus?species were characterized morphologically, microscopically and molecularly. In addition, an attempt was made to characterize the frequently occurring aflatoxigenic and non toxicAspergillus?species at molecular level along with their identification. Morphological variability was detected among isolates in regard to colony morphology, conidia colouration, exudates and reverse, colony texture and growth rate. Microscopic characteristics for the identification were conidial heads, stipe, colour and length of vesicles, shape and seriation, metulae covering, conidia size and shape.?For molecular characterization,?internal transcribed spacer (ITS)-4, a universal fungal primer was? utilized.?Differences in band patterns and number of bands obtained afterpolymerase chain reaction?(PCR) amplification?clearly differentiates?between theAspergillus?species. Results?also?reveal that only?A. flavus?showed amplification with all the three?aflatoxigenic primers?apa-2, ver-1 and omt-1,?which means that?only?A. flavus?was identified as aflatoxigenic and?other?Aspergillus?species as?non-toxigenic after PCR analysis. Hence, morphological, microscopic and molecular methods are important for the complete identification of important?Aspergillus?species and other fungi isolated from stored commodities.
机译：分析了大约82个三叶草粉末样品中不同真菌的关联。结果表明，曲霉是主要的属，有六个主要种，即A。尼日尔，黄曲霉，烟曲霉，土曲霉，nidulansand？阿姆斯洛达米。因此，这六个分离的曲霉属菌种在形态，显微镜和分子上都有特征。另外，还试图在分子水平上鉴定经常发生的黄曲霉毒素和无毒曲霉菌种，并对其进行鉴定。在菌落形态，分生孢子着色，渗出液和反向，菌落质地和生长速率方面，在分离株中检测到形态变异。鉴定的微观特征是分生孢子头，柄，颜色和长度的囊泡，形状和锯齿状，小叶覆盖，分生孢子的大小和形状。为了分子鉴定，“内部转录间隔子（ITS）-4”是一种通用的真菌引物。在聚合酶链反应（PCR）扩增后获得的条带模式和条带数量上的差异清楚地区分了曲霉属菌种。结果也仅表明了这一点。黄曲霉菌显示了所有三种黄曲霉毒素引物apa-2，ver-1和omt-1的扩增，这意味着只有A。经PCR分析后，黄酮被确定为黄曲霉毒素，其他曲霉属为非毒素。因此，形态学，显微学和分子学方法对于完整鉴定重要的曲霉菌种和从储存商品中分离出来的其他真菌很重要。

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