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Assessment of genetic diversity in quality protein maize (QPM) lines using simple sequence repeat (SSR) markers

机译:使用简单序列重复(SSR)标记评估优质蛋白玉米(QPM)品系的遗传多样性

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Maize is a primary source of energy supplement and can contribute up to 30% protein, 60% energy and 90% starch in an animal’s diet. In the present investigation,?48 microsatellite markers, spread across the maize genome were used for analyzing genetic diversity among the sixty three quality protein maize (QPM) lines, including lines developed in India and CIMMYT, Mexico. Polymorphic profiles for 37?simple sequence repeat (SSR)?loci aided in differentiating the QPM inbred lines. Using SSR procedures, the number of alleles per locus ranged from two to six, giving a total of 151 alleles for the 37 SSR?loci. The genotypes were grouped into different clusters using NTSYSpc2.11 programme. The?polymorphic information content (PIC)?value was found to be highest for the primers?bnlg1401, bnlg2136, bnlg1633?and?umc?1357 (0.96) while the lowest value was for the primer?umc 1656?(0.75) with the mean value of 0.48. From this study, the inbred line CML 142(w) is to give better combinations with CML 172, CM 161, CML 163, CLQRCYQ 281, CML 121, HQPM 5, HQPM 7, CML165 and CML 161 × CML 165 for the development of hybrids suitable for India.
机译:玉米是补充能量的主要来源,可在动物饮食中贡献高达30%的蛋白质,60%的能量和90%的淀粉。在本研究中,使用遍布玉米基因组的48个微卫星标记分析了63个优质蛋白玉米(QPM)品系(包括在印度和墨西哥CIMMYT开发的品系)之间的遗传多样性。 37个简单序列重复(SSR)位点的多态性谱有助于区分QPM自交系。使用SSR程序,每个基因座的等位基因数量从2个到6个不等,从而为37个SSR?loci总共提供了151个等位基因。使用NTSYSpc2.11程序将基因型分组为不同的簇。发现引物“ bnlg1401,bnlg2136,bnlg1633”和“ umc” 1357的“多态信息含量”(PIC)值最高(0.96),而引物“ umc 1656”(0.75)的最低值。平均值为0.48。根据这项研究,自交系CML 142(w)可以与CML 172,CM 161,CML 163,CLQRCYQ 281,CML 121,HQPM 5,HQPM 7,CML165和CML 161×CML 165更好地结合使用适合印度的混合动力车。

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