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首页> 外文期刊>African Journal of Biotechnology >Polyploidy levels of Chinese large-flower chrysanthemum determined by flow cytometry
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Polyploidy levels of Chinese large-flower chrysanthemum determined by flow cytometry

机译：流式细胞仪测定中国大花菊花的多倍性水平

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Flow cytometry was used to determine the ploidy level of 405 Chinese large-flower chrysanthemum (Chrysanthemum morifolium?Ramat.) cultivars. Sixty-three cultivars are?triploid, 175 cultivars tetraploid, 32 cultivars pentaploid, 46 cultivars hexaploid and 1 cultivar heptaploid. Forty-eight cultivars were then randomly selected for confirmation by chromosome-counting; the results are in agreement with the classification of ploidy level by flow cytometry. Most cultivars are aneuploid. The high percentage of tetraploid and triploid, instead of hexaploid in previous studies, represents the first evidence of low ploidy in large-flower chrysanthemum, which indicated a wider range of ploidy variation in this population. The results also offer further insights to the possible evolution and the regulation of flower size of this large-flower population. Additionally, the combination of flow cytometry and chromosome-counting is proved to be efficient and necessary for large-scale ploidy screening of chrysanthemum.
机译：用流式细胞仪测定405个中国大花菊花（Chrysanthemum morifolium？Ramat。）品种的倍性水平。三倍体63个，四倍体175个，五倍体32个，六倍体46个，七倍体1个。然后随机选择48个品种，通过染色体计数进行确认。结果与通过流式细胞仪对倍性水平进行分类是一致的。大多数品种是非整倍体。四倍体和三倍体的高百分比，而不是先前的研究中的六倍体，代表了大花菊花中低倍性的第一个证据，这表明该种群中倍性变化范围更大。结果也为这种大花种群的可能进化和花大小调控提供了进一步的见解。另外，流式细胞术和染色体计数的结合被证明是有效的，并且对于大规模的菊花倍性筛查是必需的。

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