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首页> 外文期刊>African Journal of Biotechnology >Isolation and identification of Metarhizium anisopliae from Chilo venosatus (Lepidoptera: Pyralidae) cadaver
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Isolation and identification of Metarhizium anisopliae from Chilo venosatus (Lepidoptera: Pyralidae) cadaver

机译：Chi虫尸体中甲状Meta的分离和鉴定

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摘要

Sugarcane stem borer,?Chilo venosatus?Walker (Lepidoptera: Pyralidae) is a significant sugarcane pest in South China. Conidia or mycelia collected from the surface of?sugarcane stem borer?cadavers were cultured. The colony morphology, mycelia and conidial yield were observed with three-agar culture media: potato dextrose agar medium (PDA), potato dextrose with 1% (w/v) peptone agar medium (PPDA), and oatmeal agar medium (OMA). 16 different isolates were identified asMetarhizium anisopliae?(Metschnikoff) based on macromorphological, micromorphological, and molecular characteristics, and PPDA was the better culture medium for vegetative growth and conidial yield (109?conidia/ml) than PDA (108conidia/ml) and OMA (108?conidia/ml). To confirm whether these isolates were pathogenic to?C. venosatus, their virulence to the?sugarcane stem borer?was tested in the laboratory. Both HS (10 isolates) and LY (6 isolates) strains were pathogenic to?C. venosatus. Several highly virulent strains were screened?in vitro?(the mortalities of the eight isolates HS1, HS6, HS7, LY2, LY3, LY6, HS3 and HS9 were from 96 to 100%), and tests for controlling the?sugarcane stem borer?were preliminarily performed?in vivo. The results show that significant (p=0.01) reductions in adult population were caused by the strains. So,?M. anisopliae?isolated from the cadavers of?C. venosatusWalker is a potential biocontrol agent against this pest in South China.?Keywords:?Metarhizium anisopliae, isolation, identification,?Chilo venosatus, culture medium, biological control.
机译：甘蔗茎bore，Chilo venosatus，Walker（鳞翅目：Pyralidae）是中国南方的重要甘蔗害虫。培养从甘蔗茎bore尸体表面收集的分生孢子或菌丝体。在三种琼脂培养基上观察菌落形态，菌丝体和分生孢子产量：马铃薯葡萄糖琼脂培养基（PDA），具有1％（w / v）蛋白ept琼脂培养基（PPDA）和燕麦琼脂培养基（OMA）的马铃薯葡萄糖。根据宏观形态，微观形态和分子特征，鉴定出16种不同的分离物为Metarhizium anisopliae？（Metschnikoff），与PDA（108 conidia / ml）和OMA相比，PPDA是营养生长和分生孢子产量（109？conidia / ml）更好的培养基（108？分生孢子/毫升）。确认这些分离株是否对C致病。 venosatus，它们对'甘蔗茎虫'的毒性在实验室中进行了测试。 HS（10株）和LY（6株）菌株均对？C致病。 venosatus。在体外筛选了几种高毒力菌株（八个分离株HS1，HS6，HS7，LY2，LY3，LY6，HS3和HS9的死亡率从96％到100％），并进行了控制甘蔗茎bore的试验。初步在体内进行。结果表明，该菌株导致成年人口显着减少（p = 0.01）。那么，M。从？C的尸体中分离出来的。在中国南方，Walker是一种潜在的防治这种病虫害的生物防治剂。关键词：金属异型甲虫，分离，鉴定，Chiolo venosatus，培养基，生物防治。

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