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首页> 外文期刊>African Journal of Biotechnology >Characterization of cucumber mosaic virus isolated from yam (Dioscorea spp.) in West Africa
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Characterization of cucumber mosaic virus isolated from yam (Dioscorea spp.) in West Africa

机译：从西非山药（Dioscorea spp。）分离的黄瓜花叶病毒的特征

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摘要

Millions of people in the West African sub-region depend on yam for food and income. In 2008, cucumber mosaic?virus (CMV), one of the most economically important plant viruses was detected in yam fields in Ghana, Benin and Togo, three of the five topmost yam producing countries in the world. Some strains of CMV are reportedly more virulent than others thus the need to characterise the strain isolated from yam. Sap inoculation of the yam strain induced systemic mosaic on?Cucumis sativus?and systemic chlorosis, necrotic lesions and leaf distortion on?Nicotiana glutinosa.Sequence analysis of the?3' end of the coat protein gene and C-terminal noncoding region?revealed 98 to 99, 93 to 98 and 78 to 79% nucleotide homology with members of the subgroups IA, IB and II, respectively. This analysis further revealed the absence of the?EcoR1 restriction site characteristic of subgroup II strains and the presence of 15 nucleotide deletions dispersed along the?C-terminal noncoding region of subgroup IA strains. At the amino acid level, the virus had 99 to 100% homology with subgroup I strains and 89% homology with subgroup II strains. Phylogenetic analysis of the amino acid confirms that the yam strain of CMV belongs to subgroup I while nucleotide sequence phylogeny confirms its placement in subgroup IA.
机译：西非次区域的数百万人依靠山药获得食物和收入。 2008年，在世界上五个山药产量最高的国家中的三个加纳，贝宁和多哥的山药田中发现了黄瓜花叶病毒（CMV），这是一种最重要的经济植物病毒。据报道，某些CMV菌株比其他菌株更具毒性，因此需要表征从山药中分离的菌株。液汁接种山药对黄瓜造成的系统性镶嵌，对烟草造成系统性萎黄病，坏死性病变和叶片变形。外壳蛋白基因的3'端和C端非编码区的序列分析显示98分别与1A，1B和II亚组的成员具有99％，93％至98％和78％至79％的核苷酸同源性。该分析进一步揭示了不存在亚组Ⅱ菌株的ΔEcoR1限制性位点特征，并且存在沿亚组IA菌株的ΔC末端非编码区分散的15个核苷酸缺失。在氨基酸水平上，该病毒与I亚组毒株具有99至100％的同源性，与II亚毒株具有89％的同源性。氨基酸的系统发育分析证实CMV的山药菌株属于I亚组，而核苷酸序列系统发育证实其位于IA亚组中。

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