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首页> 外文期刊>African Journal of Biotechnology >Genomic DNA extraction from sapwood of Pinus roxburghii for polymerase chain reaction studies
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Genomic DNA extraction from sapwood of Pinus roxburghii for polymerase chain reaction studies

机译：从松材材边材中提取基因组DNA进行聚合酶链反应研究

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摘要

A method for extraction of genomic DNA from sapwood tissues of mature tall trees ofPinus roxburghii,?where collection of needle tissues is extremely difficult has been standardized. The extracted DNA was comparable to that obtained from the needle tissue in terms of yield and purity. The yield of extracted DNA ranged from 6.98 to 19.668 μg / 100 mg tissue and A260?/ A280?ratio ranged from 1.70 to 1.87. The polymerase chain reaction (PCR) amplification of the DNA extracted from sapwood tissue using? random amplification of polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers was similar to that of DNA extracted from the needle tissues.
机译：已经标准化了一种从成熟针叶树的边材组织中提取基因组DNA的方法，该树的针叶组织很难收集。就产量和纯度而言，提取的DNA与从针头组织获得的DNA相当。提取的DNA产量为6.98至19.668μg/ 100 mg组织，A260α/ A280比率为1.70至1.87。聚合酶链反应（PCR）扩增从边材组织中提取的DNA，使用？多态性DNA（RAPD），内部简单序列重复（ISSR）和简单序列重复（SSR）标记的随机扩增与从针头组织中提取的DNA相似。

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