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首页> 外文期刊>African Journal of Biotechnology >Evaluation of genetic diversity between toxic and non toxic Jatropha curcas L. accessions using a set of simple sequence repeat (SSR) markers
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Evaluation of genetic diversity between toxic and non toxic Jatropha curcas L. accessions using a set of simple sequence repeat (SSR) markers

机译：使用一组简单序列重复（SSR）标记评估有毒和无毒麻疯树种质之间的遗传多样性

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摘要

Scepticism about Jatropha as a competitive biofuel feedstock especially on marginal soils has been growing; in fact, the jatropha-biofuel chain is risky economically and often financially unfeasible without significant government subsidies under these conditions. A valorization of the by-products and in particular of the extruded seed cake (about 70% (w/w) of the processed seed), as animal feed, currently prevented by the presence of phorbol esters (PE) toxins, could contribute to a significant improvement in the economic sustainability of the crop. Strategies for breeding improved varieties could be accelerated by DNA-based molecular marker technology. Wild Mexican accessions and accessions from other parts of the world (South America and Africa) were analyzed by 40?simple sequence repeat?(SSR) markers. SSR primers were chosen on?the grounds of their Tm, length, degree of polymorphism and specificity for toxic trait. The genetic study pointed out a high degree of similarity both within and among the non Mexican accessions. The Mexican accessions proved to be non toxic and genetically differentiated forming a well separated cluster from out of Mexico accessions. Some polymorphic loci were close correlated with the character toxicity and useful, once validated their association in segregating populations for Marker Assisted Selection (MAS).
机译：人们越来越怀疑麻疯树是一种竞争性生物燃料原料，尤其是在边缘土壤上。事实上，在这种情况下，如果没有大量的政府补贴，麻风树的生物燃料链在经济上和风险上都是不可行的。目前被佛波酯（PE）毒素阻止的副产品，尤其是膨化的种子饼（加工后的种子的约70％（w / w））作为动物饲料的增值，可能有助于大大改善了作物的经济可持续性。基于DNA的分子标记技术可以加快育种改良品种的策略。通过40个“简单序列重复”（SSR）标记分析了墨西哥野生种和来自世界其他地区（南美和非洲）的种。 SSR引物的选择基于其Tm，长度，多态性程度和毒性特征的特异性。遗传研究指出，在非墨西哥种质内和种间高度相似。墨西哥种质被证明是无毒的，并且在遗传上有所区别，与墨西哥种质形成了良好分离的簇。一些多态性位点与字符毒性密切相关并且有用，一旦证实它们在隔离群体中的关联即可进行标记辅助选择（MAS）。

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