• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Use of natural fluorescent triacyglycerols from Parinari glaberrimum to detect low lipase activity in Brassica napus seedlings
【24h】

Use of natural fluorescent triacyglycerols from Parinari glaberrimum to detect low lipase activity in Brassica napus seedlings

机译：使用来自Parinari glaberrimum的天然荧光三酰甘油检测甘蓝型油菜幼苗中的低脂肪酶活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lipases (triacylglycerol acyl hydrolass EC 3.1.1.3) are defined as enzymes that catalyse the hydrolysis of triacylglycerols, releasing long-chain fatty acids. Germinating oilseeds have been explored as a possible source of lipases for biotechnological processing of oils and fats. However, purification and sensitive assays to detect a true lipase activity in plant cellular homogenates and culture media are required. The main aim of this study was to design a convenient, specific, sensitive and continuous lipase activity assay using natural long-chain triacylglycerols (TAGs). Oil was ex tracted from?Parinari glaberrimum?seed kernels and the pu rified TAGs were used as a substrate for detecting low levels of lipase activities. An increase in the fluorescence was observed, which is due to the parinaric acid released by various lipase activities. This increase in the fluorescence intensity is linear with time and proportional to the amount of lipase added. This new method, performed under non-oxidative conditions, was applied successfully to detect low lipase levels in crude protein extracts from plant seeds.
机译：脂肪酶（三酰基甘油酰基水解酶EC 3.1.1.3）定义为催化三酰基甘油水解并释放长链脂肪酸的酶。人们已经探索发芽的油料种子是油脂生物技术加工中脂肪酶的一种可能来源。但是，需要进行纯化和灵敏的检测以检测植物细胞匀浆和培养基中真正的脂肪酶活性。这项研究的主要目的是设计一种使用天然长链三酰基甘油（TAGs）的便捷，特异性，灵敏和连续的脂肪酶活性测定方法。从Parinari glaberrimum-seed籽粒中提取油，纯化的TAGs作为底物来检测低水平的脂肪酶活性。观察到荧光的增加，这是由于各种脂肪酶活性所释放的二十二烷酸。荧光强度的这种增加与时间成线性关系，并且与脂肪酶的添加量成正比。这种在非氧化条件下进行的新方法已成功应用于检测植物种子粗蛋白提取物中的低脂肪酶水平。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号